Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae.
Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae.
复制标题
二斑叶螨 (Tetranychus urticae) 中沃尔巴克氏体反应性 microRNA 的鉴定
DOI：
10.1186/1471-2164-15-1122
复制
发表时间：
2014-12-16
期刊：
影响因子：
4.4
通讯作者：
Hong XY
中科院分区：
文献类型：
--
作者：
Rong X;Zhang YK;Zhang KJ;Hong XY
两斑蜘蛛Tetranychus urticae被沃尔巴克氏菌感染，沃尔巴克氏菌具有操纵宿主繁殖和适应的能力。MicroRNAs(MiRNAs)是一种小的非编码RNA，参与许多生物学过程，如发育、繁殖和宿主与病原体的相互作用。虽然在其他昆虫系统中观察到miRNA参与了Wolbachia-宿主的相互作用，但它在两点蜘蛛体内的作用尚未完全破译。构建了感染和未感染麻风菌的两性小RNA文库(共4个文库)。通过整合来自相同样本的mRNA数据，预测了差异表达的miRNAs的靶基因。然后对目的基因进行GO和通径分析。文库比较表明，沃尔巴克氏菌感染显著调控雌性的91个miRNAs和雄性的20个miRNAs，而在Wolbachia感染的文库中，miRNAs总体上受到抑制。MiRNA和mRNA数据的比较预测，差异表达的miRNAs负调控雌性的90个mRNAs和雄性的9个mRNAs。对靶基因的分析表明，沃尔巴克氏反应miRNAs调控的基因包括鞘磷脂代谢、溶酶体功能、细胞凋亡和脂质运输，以及雌性动物的生殖。MiRNA和mRNA数据的比较有助于识别参与Wolbachia-宿主相互作用的miRNAs和miRNA靶基因。本研究确定的分子靶点在进一步的功能研究中应该是有用的。
The two-spotted spider mite, Tetranychus urticae, is infected with Wolbachia, which have the ability to manipulate host reproduction and fitness. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in many biological processes such as development, reproduction and host-pathogen interactions. Although miRNA was observed to involve in Wolbachia-host interactions in the other insect systems, its roles have not been fully deciphered in the two-spotted spider mite. Small RNA libraries of infected and uninfected T. urticae for both sexes (in total four libraries) were constructed. By integrating the mRNA data originated from the same samples, the target genes of the differentially expressed miRNAs were predicted. Then, GO and pathway analyses were performed for the target genes. Comparison of libraries showed that Wolbachia infection significantly regulated 91 miRNAs in females and 20 miRNAs in males, with an overall suppression of miRNAs in Wolbachia-infected libraries. A comparison of the miRNA and mRNA data predicted that the differentially expressed miRNAs negatively regulated 90 mRNAs in females and 9 mRNAs in males. An analysis of target genes showed that Wolbachia-responsive miRNAs regulated genes with function in sphingolipid metabolism, lysosome function, apoptosis and lipid transporting in both sexes, as well as reproduction in females. Comparisons of the miRNA and mRNA data can help to identify miRNAs and miRNA target genes involving in Wolbachia-host interactions. The molecular targets identified in this study should be useful in further functional studies.
登录
查看更多内容
影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
3.6
作者：
Cao, Wenlei;Aghajanian, Haig K.;Gerton, George L.
通讯作者：
Gerton, George L.
影响因子：
3.3
作者：
Freitak D;Knorr E;Vogel H;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
6.7
作者：
Landmann F;Orsi GA;Loppin B;Sullivan W
通讯作者：
Sullivan W