Canine babesiosis in northern Portugal and molecular characterization of vector-borne co-infections.
Canine babesiosis in northern Portugal and molecular characterization of vector-borne co-infections.
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DOI：
10.1186/1756-3305-3-27
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发表时间：
2010-04-08
影响因子：
3.2
通讯作者：
Baneth G
中科院分区：
文献类型：
--
作者：
Cardoso L;Yisaschar-Mekuzas Y;Rodrigues FT;Costa A;Machado J;Diz-Lopes D;Baneth G
由节肢动物（包括蜱虫和白蛉）传播的原生动物和细菌可能会引起多种犬类媒介传播疾病。狗可以同时或相继感染多种病原体。已知由犬巴贝斯虫和犬巴贝斯虫引起的犬巴贝斯虫病发生在葡萄牙。本研究通过血涂片检查、PCR 和 DNA 核苷酸测序评估了巴贝虫属的存在。来自葡萄牙北部的 45 只临床怀疑患有巴贝斯虫病的狗体内存在利什曼原虫、无形体/埃利希体和肝细胞共感染因子。 44 只狗 (98%) 感染了犬双歧杆菌，一只感染了犬双歧杆菌。在 9 只动物 (20%) 中检测到双重感染。八只狗被发现感染了两种媒介传播的病原体：其中六只感染了犬双歧杆菌和婴儿利什曼原虫；一种是犬布鲁氏菌（B. canis canis canis）和犬埃里希体（Ehrlichia canis）；一种是犬双歧杆菌和犬肝原虫。另一只狗感染了三种媒介传播的病原体：犬芽孢杆菌、犬埃球菌和婴儿乳杆菌。总体而言，在 45 只患有巴贝西虫病的狗中，有 7 只 (16%) 发现了婴儿乳杆菌，2 只 (4%) 发现了犬嗜血杆菌，1 只 (2%) 发现了犬嗜血杆菌。 45 例犬巴贝西虫病病例中，近 90% 是在十月（18%）、十一月（27%）、十二月（20%）、二月（13%）和三月（9%）等较冷月份诊断出来的。 2月、3月、4月、5月、10月和11月发现了混合感染。 44 只感染犬小孢子虫的狗中，有 22 只 (50%) 被发现感染了蜱虫，包括革蜱属 (Dermacentor spp.)、硬蜱属 (Ixodes spp.)。和血扇头扇贝。死亡率（9%）包括两只同时感染的狗自然死亡和两只单一感染的狗被安乐死。犬巴贝斯虫是葡萄牙北部犬巴贝斯虫病的主要病原体。对巴贝虫属的敏感性较高。与血涂片观察相比，通过PCR检测获得检测结果。百分之二十的狗同时感染了婴儿乳杆菌、犬艾美耳球菌或犬幽门螺杆菌。此外，这是对来自葡萄牙北部的狗中犬嗜血杆菌的首次分子鉴定。
Protozoa and bacteria transmitted by arthropods, including ticks and phlebotomine sand flies, may cause a wide range of canine vector-borne diseases. Dogs can be simultaneously or sequentially infected with multiple pathogens. Canine babesiosis caused by Babesia canis canis and Babesia canis vogeli is known to occur in Portugal. This study assessed, by means of blood smear examination, PCR and DNA nucleotide sequencing, the presence of Babesia spp. and co-infecting agents Leishmania, Anaplasma/Ehrlichia and Hepatozoon in 45 dogs from northern Portugal clinically suspected of babesiosis. Forty-four dogs (98%) had infection with B. canis canis and one with B. canis vogeli. Co-infections were detected in nine animals (20%). Eight dogs were found infected with two vector-borne agents: six with B. canis canis and Leishmania infantum; one with B. canis canis and Ehrlichia canis; and one with B. canis canis and Hepatozoon canis. Another dog was infected with three vector-borne pathogens: B. canis vogeli, E. canis and L. infantum. Overall, L. infantum was found in seven (16%), E. canis in two (4%), and H. canis in one (2%) out of the 45 dogs with babesiosis. Almost 90% of the 45 cases of canine babesiosis were diagnosed in the colder months of October (18%), November (27%), December (20%), February (13%) and March (9%). Co-infections were detected in February, March, April, May, October and November. Twenty-two (50%) out of 44 dogs infected with B. canis were found infested by ticks including Dermacentor spp., Ixodes spp. and Rhipicephalus sanguineus. Mortality (9%) included two co-infected dogs that died spontaneously and two with single infections that were euthanized. Babesia canis canis is the main etiological agent of canine babesiosis in northern Portugal. A higher sensitivity of Babesia spp. detection was obtained with PCR assays, compared to the observation of blood smears. Twenty percent of the dogs were co-infected with L. infantum, E. canis or H. canis. Furthermore, this is the first molecular identification of H. canis in dogs from northern Portugal.
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DOI：
10.2460/javma.2005.227.942
发表时间：
2005-09-15
影响因子：
1.9
作者：
Birkenheuer, AJ;Correa, MT;Breitschwerdt, EB
通讯作者：
Breitschwerdt, EB
影响因子：
2.6
作者：
Inokuma, HA;Okuda, M;Onishi, T
通讯作者：
Onishi, T
影响因子：
2.6
作者：
Criado, A.;MartineZ, J.;Irwin, P. J.
通讯作者：
Irwin, P. J.
DOI：
10.1016/s0195-5616(03)00039-1
发表时间：
2003-07-01
影响因子：
1.9
作者：
Boozer, AL;Macintire, DK
通讯作者：
Macintire, DK
影响因子：
2.6
作者：
Inokuma, H;Yoshizaki, Y;Hirakawa, M
通讯作者：
Hirakawa, M