Extraction of a Novel Cold-Water-Soluble Polysaccharide from Astragalus membranaceus and Its Antitumor and Immunological Activities.
Extraction of a Novel Cold-Water-Soluble Polysaccharide from Astragalus membranaceus and Its Antitumor and Immunological Activities.
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DOI：
10.3390/molecules23010062
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发表时间：
2017-12-28
期刊：
影响因子：
--
通讯作者：
Liu HP
中科院分区：
文献类型：
--
作者：
Liu AJ;Yu J;Ji HY;Zhang HC;Zhang Y;Liu HP
黄芪多糖已受到广泛的研究和关注，但很少有报道使用冷水（4 °C）提取这些多糖。本研究采用DEAE-cellulose 52阴离子交换柱和Sephadex G-100柱从黄芪中分离得到一种新型冷水可溶性多糖（cAMPs-1A），其碳水化合物含量为92.00%。我们的UV、傅里叶变换红外光谱（FTIR）、高效凝胶渗透色谱和离子色谱分析结果表明，分子量为1.23 × 104 Da的cAMPs-1A的单糖组成为岩藻糖、阿拉伯糖、半乳糖、葡萄糖和木糖，摩尔比分别为0.01：0.06：0.20：1.00：0.06。UV光谱法未检测到cAMPs-1A中的蛋白质和核酸。我们使用FTIR分析来表征cAMPs-1A中的α-d-吡喃型构型。此外，我们在体内进行动物实验，以评估cAMPs-1A的抗肿瘤和免疫调节作用。结果表明，cAMPs-1A在75、150和300 mg/kg剂量下对肿瘤生长有明显的抑制作用，抑制率分别为20.53%、36.50%和44.49%。此外，cAMPs-1A还能有效保护荷瘤小鼠的免疫器官，促进巨噬细胞胞饮，提高外周血淋巴细胞亚群的比例。这些发现表明多糖cAMPs-1A具有作为天然抗肿瘤剂的潜在应用。
The polysaccharides of Astragalus membranaceus have received extensive study and attention, but there have been few reports on the extraction of these polysaccharides using cold water (4 °C). In this study, we fractionated a novel cold-water-soluble polysaccharide (cAMPs-1A) from Astragalus membranaceus with a 92.00% carbohydrate content using a DEAE-cellulose 52 anion exchange column and a Sephadex G-100 column. Our UV, Fourier-transform infrared spectroscopy (FTIR), high-performance gel permeation chromatography, and ion chromatography analysis results indicated the monosaccharide composition of cAMPs-1A with 1.23 × 104 Da molecular weight to be fucose, arabinose, galactose, glucose, and xylose, with molar ratios of 0.01:0.06:0.20:1.00:0.06, respectively. The UV spectroscopy detected no protein and nucleic acid in cAMPs-1A. We used FTIR analysis to characterize the α-d-pyranoid configuration in cAMPs-1A. In addition, we performed animal experiments in vivo to evaluate the antitumor and immunomodulatory effects of cAMPs-1A. The results suggested that cAMPs-1A oral administration could significantly inhibit tumor growth with the inhibitory rate of 20.53%, 36.50% and 44.49%, respectively, at the dosage of 75,150, and 300 mg/kg. Moreover, cAMPs-1A treatment could also effectively protect the immune organs, promote macrophage pinocytosis, and improve the percentages of lymphocyte subsets in the peripheral blood of tumor-bearing mice. These findings demonstrate that the polysaccharide cAMPs-1A has an underlying application as natural antitumor agents.
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影响因子：
2.9
作者：
Barbosa, Helder;Slater, Nigel K. H.;Marcos, Joao C.
通讯作者：
Marcos, Joao C.
影响因子：
2.9
作者：
BITTER, T;MUIR, HM
通讯作者：
MUIR, HM
DOI：
10.1039/jr9540000171
发表时间：
1954-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY
影响因子：
--
作者：
BARKER, SA;BOURNE, EJ;WHIFFEN, DH
通讯作者：
WHIFFEN, DH
影响因子：
2.6
作者：
Liu, Anjun;Liu, Dongyue;Zhang, Hong
通讯作者：
Zhang, Hong
影响因子：
2.7
作者：
ALAM, N;GUPTA, PC
通讯作者：
GUPTA, PC