Protein-assisted conjugated polymer microarray: Fabrication and sensing applications

Protein-assisted conjugated polymer microarray: Fabrication and sensing applications
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蛋白质辅助共轭聚合物微阵列:制造和传感应用

DOI:
10.1007/s11434-013-6025-6
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发表时间:
2013-08
影响因子:
--
通讯作者:
Wang, Shu
Wang, Shu
中科院分区:
--
文献类型:
--
作者:
Lv, Fengting;Feng, Xuli;Liu, Libing;Wang, Shu

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本文提出了一种新的蛋白质辅助制备共轭聚合物微阵列的方法。本工作中采用的水溶性阳离子共轭聚合物能够吸收510 nm处的光,这使得它与各种商业微阵列扫描仪兼容。结果表明,蛋白质辅助的偶联聚合物微阵列显示出更高的荧光信号和更好的稳定性相比,没有蛋白质的情况下。该偶联聚合物微阵列可用于苦味酸(PA)的灵敏检测。我们的方法的一个主要优点是它的简单性和化学连接之间的共轭聚合物和微阵列基板是不需要的。考虑到共轭聚合物微阵列制备的简单性,可以预期,新的传感平台将通过采用这种通用的方法构建。
A new strategy is performed to fabricate conjugated polymer microarray with the assistance of protein in this work. The water-soluble cationic conjugated polymer employed in the present work is capable of absorbing light at 510 nm, which makes it compatible with a variety of commercial microarray scanners. It is demonstrated that the protein-assisted conjugated polymer microarray exhibits higher fluorescence signal and better stability in comparison with the case without protein. The conjugated polymer microarray can be used for sensitive detection of picric acid (PA). A major advantage of our approach is its simplicity and chemical linking is not required between the conjugated polymer and microarray substrate. Considering the simplicity of the preparation of the conjugated polymer microarray, it is anticipated that novel sensing platforms will be constructed by employing this versatile method.
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