Investigating the Molecular Genetic Basis of Cytoplasmic Sex Determination Caused by Wolbachia Endosymbionts in Terrestrial Isopods.
Investigating the Molecular Genetic Basis of Cytoplasmic Sex Determination Caused by Wolbachia Endosymbionts in Terrestrial Isopods.
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研究由陆生等异类中沃尔巴氏菌内共生体引起的细胞质性别测定的分子遗传基础。
DOI：
10.3390/genes9060290
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发表时间：
2018-06-08
期刊：
影响因子：
3.5
通讯作者：
Cordaux R
中科院分区：
文献类型：
--
作者：
Badawi M;Moumen B;Giraud I;Grève P;Cordaux R
在动物中，雄性和雌性之间的性别差异通常由性染色体决定。或者，性别也可以由垂直传播的细胞内微生物内共生体决定。最著名的细胞质性别操纵内共生体是沃尔巴克氏体，它可以，例如，在陆地等足动物Armadillidium vulgare中，将遗传雄性雌性化为表型雌性。然而，细胞质性别决定的分子遗传基础是未知的。筛选Wolbachia株wVulC诱导A. vulgare中，我们对来自Cylisticus convexus的Wolbachia菌株wCon的基因组进行了测序，该Wolbachia菌株是已知与wVulC最密切相关的Wolbachia菌株，其不诱导雌性化，并将其与wVulC基因组进行了比较。然后，我们在A. vulgare和异源寄主C.凸的我们确定了一组35女性化候选基因显示差异表达在主机性发育。有趣的是，35个基因中有27个存在于f元件中，f元件是一个雌性化的沃尔巴克氏体基因组的一部分，水平转移到A.与女性的性别决定有关。假设Wolbachia和f元件导致雌性化的分子遗传基础相同，则这27个基因可能是A.携带f元素的粗俗女性。
In animals, sexual differences between males and females are usually determined by sex chromosomes. Alternatively, sex may also be determined by vertically transmitted intracellular microbial endosymbionts. The best known cytoplasmic sex manipulative endosymbiont is Wolbachia which can, for instance, feminize genetic males into phenotypic females in the terrestrial isopod Armadillidium vulgare. However, the molecular genetic basis of cytoplasmic sex determination is unknown. To identify candidate genes of feminization induced by Wolbachia strain wVulC from A. vulgare, we sequenced the genome of Wolbachia strain wCon from Cylisticus convexus, the most closely related known Wolbachia strain to wVulC that does not induce feminization, and compared it to the wVulC genome. Then, we performed gene expression profiling of the 216 resulting wVulC candidate genes throughout host developmental stages in A. vulgare and the heterologous host C. convexus. We identified a set of 35 feminization candidate genes showing differential expression during host sexual development. Interestingly, 27 of the 35 genes are present in the f element, which is a piece of a feminizing Wolbachia genome horizontally transferred into the nuclear genome of A. vulgare and involved in female sex determination. Assuming that the molecular genetic basis of feminization by Wolbachia and the f element is the same, the 27 genes are candidates for acting as master sex determination genes in A. vulgare females carrying the f element.
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影响因子：
16.6
作者：
Bonneau M;Atyame C;Beji M;Justy F;Cohen-Gonsaud M;Sicard M;Weill M
通讯作者：
Weill M
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Abu Kwaik, Yousef
影响因子：
3.7
作者：
Badawi, Myriam;Greve, Pierre;Cordaux, Richard
通讯作者：
Cordaux, Richard