Data for iTRAQ-based quantitative proteomics analysis of Brassica napus leaves in response to chlorophyll deficiency.
Data for iTRAQ-based quantitative proteomics analysis of Brassica napus leaves in response to chlorophyll deficiency.
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DOI：
10.1016/j.dib.2014.10.004
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发表时间：
2015-03
期刊：
影响因子：
1.2
通讯作者：
Guan, Rong Zhan
中科院分区：
文献类型：
--
作者：
Chu, Pu;Yan, Gui Xia;Yang, Qing;Zhai, Li Na;Zhang, Cheng;Zhang, Feng Qi;Guan, Rong Zhan
叶绿素(Chl)在光合作用过程中起着重要的捕光和能量传递作用。在这里，我们提供了利用iTRAQ方法对叶绿素缺乏的油菜突变体cde1及其相应的野生型进行比较蛋白质组分析的数据(Pu Chu等人，2014)。计算所鉴定蛋白质的长度和数量、质量和序列覆盖率的分布，并分析重复序列的可重复性。在甘蓝型油菜叶片中共鉴定出443个差异表达蛋白质，其中向下积累的蛋白质有228个，主要涉及光合作用、卟啉和叶绿素代谢、次生代谢产物的生物合成、固碳等；向上积累的蛋白质有215个，主要集中在剪接体、mRNA监测和RNA降解等方面。
The essential pigment chlorophyll (Chl) plays important roles in light harvesting and energy transfer during photosynthesis. Here we present the data from a comparative proteomic analysis of chlorophyll-deficient Brassica napus mutant cde1 and its corresponding wild-type using the iTRAQ approach (Pu Chu et al., 2014). The distribution of length and number of peptides, mass and sequence coverage of proteins identified was calculated, and the repeatability of the replicates was analyzed. A total of 443 differentially expressed proteins were identified in B. napus leaves, including 228 down-accumulated proteins mainly involved in photosynthesis, porphyrin and chlorophyll metabolism, biosynthesis of secondary metabolites, carbon fixation and 215 up-accumulated proteins that enriched in the spliceosome, mRNA surveillance and RNA degradation.
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