Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging.
Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging.
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DOI：
10.1021/acsami.2c02551
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发表时间：
2022-05-11
影响因子：
9.5
通讯作者：
Rosenzweig, Zeev
中科院分区：
文献类型：
--
作者：
Riahin, Connor;Meares, Adam;Esemoto, Nopondo N.;Ptaszek, Marcin;LaScola, Michael;Pandala, Narendra;Lavik, Erin;Yang, Mengran;Stacey, Gary;Hu, Dehong;Traeger, Jeremiah C.;Orr, Galya;Rosenzweig, Zeev
关键词：
近红外（NIR）荧光半导体聚合物点（Pdots）由于其特殊的化学和光物理性质，在荧光成像领域显示出巨大的潜力。本文介绍了一种发射峰波长可控、可调谐的近红外发光点的合成方法。采用荧光Pdots中常用的寄主聚合物聚[（9,9-二辛基氟芴基-2,7-二基）-羟基-co-（1,4-苯并-(2,1 ',3)-噻二唑）]（PFBT）与一系列具有不同官能团的氯和细菌氯掺杂制备Pdots。氯和细菌氯是理想的掺杂剂，由于它们的高疏水性，这使得它们不能在水生物介质中用作分子探针，但另一方面，当它们掺杂到Pdots中时，可以防止它们泄漏。此外，氯和细菌氯具有狭窄的深红色至近红外发射带，并且可用于修饰其分子结构的广泛合成修饰可以预测和系统地调节其发射。透射电子显微镜（TEM）和动态光散射（DLS）测量表明，氯和细菌氯掺杂的Pdots接近球形，平均直径为46±12 nm。PFBT与掺杂氯或细菌氯之间的有效能量转移将PFBT施主发射降低到接近基线水平，并增加了作为受体的掺杂染料的发射。根据掺杂染料的不同，氯和细菌氯掺杂的Pdots显示出640 ~ 820 nm的窄发射带。本文通过制备不同发射峰波长的Pdots，利用它们在宽视场荧光显微镜下观察多个靶标，将它们与二抗结合，并确定二抗体共轭Pdots与植物细胞中一抗标记受体的结合，证明了系统氯和细菌氯合成方法的效用。此外，氯和细菌氯掺杂的Pdots显示出闪烁行为，可以使其用于超分辨率成像方法，如STORM。
Near-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) have shown great potential for fluorescence imaging due to their exceptional chemical and photophysical properties. This paper describes the synthesis of NIR emitting Pdots with great control and tunability of emission peak wavelength. The Pdots were prepared by doping poly [(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-(2,1’,3)-thiadiazole)] (PFBT), a semiconducting polymer commonly used as a host polymer in luminescent Pdots, with a series of chlorins and bacteriochlorins with varying functional groups. Chlorins and bacteriochlorins are ideal dopants due to their high hydrophobicity, which precludes their use as molecular probes in aqueous biological media but on the other hand prevents their leakage when doped into Pdots. Additionally, chlorins and bacteriochlorins have narrow deep-red to NIR emission bands and the wide array of synthetic modifications available for modifying their molecular structure enables tuning their emission predictably and systematically. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements show the chlorin and bacteriochlorin-doped Pdots to be nearly spherical with an average diameter of 46±12 nm. Efficient energy transfer between PFBT and the doped chlorins or bacteriochlorins decreases the PFBT donor emission to near baseline level and increases the emission of the doped dyes that serve as acceptors. The chlorin and bacteriochlorin-doped Pdots show narrow emission bands ranging from 640 nm to 820 nm depending on the doped dye. The paper demonstrates the utility of the systematic chlorin and bacteriochlorin synthesis approach by preparing Pdots of varying emission peak wavelength, utilizing them to visualize multiple targets using wide field fluorescence microscopy, binding them to secondary antibodies, and determining the binding of secondary antibody-conjugated Pdots to primary antibody-labeled receptors in plant cells. Additionally, the chlorin and bacteriochlorin doped Pdots show a blinking behavior that could enable their use in super resolution imaging methods like STORM.
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影响因子：
10.4
作者：
Hardman R
通讯作者：
Hardman R
影响因子：
14
作者：
Li J;Rao J;Pu K
通讯作者：
Pu K
影响因子：
1.3
作者：
Murray, CB;Sun, SH;Kagan, CR
通讯作者：
Kagan, CR
DOI：
10.1161/01.atv.0000070546.16946.3a
发表时间：
2003-07-01
影响因子：
8.7
作者：
Frank, PG;Woodman, SE;Lisanti, MP
通讯作者：
Lisanti, MP
基于 BODIPY 的供体/供体-受体系统：实现具有窄而强吸收特性的高效长波长可激发近红外聚合物点
影响因子：
16.6
作者：
Chen, Lei;Chen, Dandan;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.