An experimental model of meningoencephalomyelitis by Rocio flavivirus in BALB/c mice: inflammatory response, cytokine production, and histopathology.
An experimental model of meningoencephalomyelitis by Rocio flavivirus in BALB/c mice: inflammatory response, cytokine production, and histopathology.
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DOI：
10.4269/ajtmh.2011.10-0246
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Figueiredo LT
中科院分区：
文献类型：
--
作者：
de Barros VE;Saggioro FP;Neder L;de Oliveira França RF;Mariguela V;Chávez JH;Penharvel S;Forjaz J;da Fonseca BA;Figueiredo LT
罗西奥病毒（ROCV）是一种黄病毒，可能由库蚊传播，在自然界中作为野生鸟类的人畜共患病而存在。罗西奥病毒在1973年至1980年期间在巴西东南海岸的里贝拉谷引起了严重脑炎的人类流行。本次疫情发生后，报告了罗氏病毒传播的血清学证据，公共卫生当局担心巴西会再次出现罗氏病毒疫情。我们在Balb/C年轻成年小鼠实验模型中对中枢神经系统ROCV病的发病机制和生理病理进行了研究。动物腹腔感染ROCV，感染后0 ~ 9 d随访，全部死亡。采集感染动物神经组织标本进行免疫组织化学和分子生物学分析。我们观察了病毒在中枢神经系统的作用，Th1和Th2细胞因子引起的炎症变化，以及神经元变性和细胞凋亡对神经组织的最终不可逆损伤。这些发现有助于更好地了解ROCV和其他黄病毒引起的人脑膜脑脊髓炎的发病机制和生理病理。
Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses.
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