A chrysanthemum heat shock protein confers tolerance to abiotic stress.

A chrysanthemum heat shock protein confers tolerance to abiotic stress.
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菊花热休克蛋白赋予对非生物胁迫的耐受性

DOI:
10.3390/ijms15035063
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发表时间:
2014-03-21
影响因子:
5.6
通讯作者:
Chen S
Chen S
中科院分区:
生物学2区
文献类型:
--
作者:
Song A;Zhu X;Chen F;Gao H;Jiang J;Chen S

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热休克蛋白与抵御各种非生物胁迫有关。本文报道了菊花中HSP70家族的一个基因的分离。该基因命名为CgHSP70,编码一个647个残基的多肽,分子量为70.90 kDa,等电点为5.12。亚细胞定位分析表明,该产物沉积在胞浆和胞核中。构建表达CgHSP70的拟南芥植株的表现表明,CgHSP70基因增强了对高温、干旱和盐分的耐性。当CgHSP70在菊花中稳定过表达时,植株表现出过氧化物酶(POD)活性增加,脯氨酸含量增加,丙二醛(MDA)含量下降。在高温、干旱或盐碱胁迫后,转基因植株恢复能力较好,表现出CgHSP70的正效应。
Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that the cDNA product is deposited in the cytoplasm and nucleus. The performance of Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated that the gene enhances tolerance to heat, drought and salinity. When CgHSP70 was stably over-expressed in chrysanthemum, the plants showed an increased peroxidase (POD) activity, higher proline content and inhibited malondialdehyde (MDA) content. After heat stress, drought or salinity the transgenic plants were better able to recover, demonstrating CgHSP70 positive effect.
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