Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birds.
Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birds.
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DOI：
10.1186/1471-2148-8-81
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发表时间：
2008-03-09
影响因子：
3.4
通讯作者：
Baker AJ
中科院分区：
文献类型：
--
作者：
Tavares ES;Baker AJ
使用标准化COI序列的生命DNA条形码被提议作为物种鉴定系统，并作为检测推定新物种的方法。先前在鸟类中的测试表明，在约94%的情况下，个体可以正确地分配到物种，并建议10×平均种内差异的阈值来检测潜在的新物种。然而，这些测试受到批评，因为它们基于单一的母系遗传基因而不是多个核基因，没有比较遗传学上鉴定的姐妹物种，因此可能高估了DNA条形码在鉴定物种方面的功效。为了测试DNA条形码的效力，我们比较了来自10个鸟类目的多基因同源性中鉴定的60个姐妹物种对中的~650 bp的COI。在所有对中，每个物种的个体在邻接（NJ）树中是单系的，并且每个物种具有将它们与其姐妹物种区分开的固定突变差异。因此，个人被正确地分配到物种使用统计结合框架。在已知的姐妹种中，基于两个谱系中偶然发生的相互单系性的分类学独特性的结合测试得到了验证，并用于识别代表推定物种的最近分离的谱系。这种方法避免了使用一个通用的距离截止，这是无效的时间变化，以共同祖先的姐妹物种和进化速度。通过COI序列中固定诊断替换的条形码，可以可靠地识别鸟类的密切相关的姐妹物种，验证基于聚结的统计检验的相互单系分类学的独特性。与最近的批评相反，单一DNA条形码是一种快速发现集合种群内单系谱系的方法，可能代表未发现的神秘物种，正如统一物种概念所设想的那样。这确定了一个较小的谱系，也可以独立测试的物种状态与多个核基因的方法和其他表型特征。
DNA barcoding of life using a standardized COI sequence was proposed as a species identification system, and as a method for detecting putative new species. Previous tests in birds showed that individuals can be correctly assigned to species in ~94% of the cases and suggested a threshold of 10× mean intraspecific difference to detect potential new species. However, these tests were criticized because they were based on a single maternally inherited gene rather than multiple nuclear genes, did not compare phylogenetically identified sister species, and thus likely overestimated the efficacy of DNA barcodes in identifying species. To test the efficacy of DNA barcodes we compared ~650 bp of COI in 60 sister-species pairs identified in multigene phylogenies from 10 orders of birds. In all pairs, individuals of each species were monophyletic in a neighbor-joining (NJ) tree, and each species possessed fixed mutational differences distinguishing them from their sister species. Consequently, individuals were correctly assigned to species using a statistical coalescent framework. A coalescent test of taxonomic distinctiveness based on chance occurrence of reciprocal monophyly in two lineages was verified in known sister species, and used to identify recently separated lineages that represent putative species. This approach avoids the use of a universal distance cutoff which is invalidated by variation in times to common ancestry of sister species and in rates of evolution. Closely related sister species of birds can be identified reliably by barcodes of fixed diagnostic substitutions in COI sequences, verifying coalescent-based statistical tests of reciprocal monophyly for taxonomic distinctiveness. Contrary to recent criticisms, a single DNA barcode is a rapid way to discover monophyletic lineages within a metapopulation that might represent undiscovered cryptic species, as envisaged in the unified species concept. This identifies a smaller set of lineages that can also be tested independently for species status with multiple nuclear gene approaches and other phenotypic characters.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
DOI：
10.1111/j.1471-8286.2006.01428.x
发表时间：
2006-12-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Ivanova, Natalia V.;Dewaard, Jeremy R.;Hebert, Paul D. N.
通讯作者：
Hebert, Paul D. N.
影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
影响因子：
6.5
作者：
De Queiroz, Kevin
通讯作者：
De Queiroz, Kevin
影响因子：
6.5
作者：
FREEMAN, S;ZINK, RM
通讯作者：
ZINK, RM