Adult microbiota-deficient mice have distinct dendritic morphological changes: differential effects in the amygdala and hippocampus.
Adult microbiota-deficient mice have distinct dendritic morphological changes: differential effects in the amygdala and hippocampus.
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DOI：
10.1111/ejn.13291
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Cryan JF
中科院分区：
文献类型：
--
作者：
Luczynski P;Whelan SO;O'Sullivan C;Clarke G;Shanahan F;Dinan TG;Cryan JF
越来越多的证据表明，微生物区系与大脑和行为的调节有关。无菌小鼠(GF；天生缺乏微生物区系)表现出压力荷尔蒙信号和焦虑样行为的改变，以及社会认知方面的缺陷。尽管肠道微生物区系影响应激反应和行为能力的潜在机制仍不清楚，但许多证据表明杏仁核和海马体可能是目标。因此，这项研究的目的是确定扁桃体和海马区的体积和树突形态在GF和常规定植(CC)小鼠中是否有所不同。体积估计显示，与CC小鼠相比，GF组的杏仁核和海马区显著扩大。我们还研究了GF状态对杏仁基底外侧核(BLA)和腹侧海马区单个神经元水平的影响。在BLA，GF小鼠的间质神经元和锥体神经元呈树突状肥大。GF小鼠的BLA锥体神经元有更多的细刺、短刺和蘑菇刺。相比之下，GF组小鼠的腹侧海马锥体神经元较短，分枝较少，且有较少的粗刺和蘑菇刺。与对照组相比，GF组小鼠的齿状颗粒细胞分枝较少，但在脊柱密度方面没有差异。这些发现表明，微生物群是杏仁核和海马体正常大体形态和超微结构所必需的，这种神经重建可能有助于在GF小鼠中观察到适应不良的应激反应和行为特征。
Increasing evidence implicates the microbiota in the regulation of brain and behaviour. Germ‐free mice (GF; microbiota deficient from birth) exhibit altered stress hormone signalling and anxiety‐like behaviours as well as deficits in social cognition. Although the mechanisms underlying the ability of the gut microbiota to influence stress responsivity and behaviour remain unknown, many lines of evidence point to the amygdala and hippocampus as likely targets. Thus, the aim of this study was to determine if the volume and dendritic morphology of the amygdala and hippocampus differ in GF versus conventionally colonized (CC) mice. Volumetric estimates revealed significant amygdalar and hippocampal expansion in GF compared to CC mice. We also studied the effect of GF status on the level of single neurons in the basolateral amygdala (BLA) and ventral hippocampus. In the BLA, the aspiny interneurons and pyramidal neurons of GF mice exhibited dendritic hypertrophy. The BLA pyramidal neurons of GF mice had more thin, stubby and mushroom spines. In contrast, the ventral hippocampal pyramidal neurons of GF mice were shorter, less branched and had less stubby and mushroom spines. When compared to controls, dentate granule cells of GF mice were less branched but did not differ in spine density. These findings suggest that the microbiota is required for the normal gross morphology and ultrastructure of the amygdala and hippocampus and that this neural remodelling may contribute to the maladaptive stress responsivity and behavioural profile observed in GF mice.
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早期生活节目和神经发育障碍。
影响因子：
10.6
作者：
Bale, Tracy L.;Baram, Tallie Z.;Brown, Alan S.;Goldstein, Jill M.;Insel, Thomas R.;McCarthy, Margaret M.;Nemeroff, Charles B.;Reyes, Teresa M.;Simerly, Richard B.;Susser, Ezra S.;Nestler, Eric J.
通讯作者：
Nestler, Eric J.
影响因子：
10.6
作者：
Abazyan, Bagrat;Nomura, Jun;Kannan, Geetha;Ishizuka, Koko;Tamashiro, Kellie L.;Nucifora, Frederick;Pogorelov, Vladimir;Ladenheim, Bruce;Yang, Chunxia;Krasnova, Irina N.;Cadet, Jean Lud;Pardo, Carlos;Mori, Susumu;Kamiya, Atsushi;Vogel, Michael W.;Sawa, Akira;Ross, Christopher A.;Pletnikov, Mikhail V.
通讯作者：
Pletnikov, Mikhail V.
影响因子：
1.9
作者：
Deacon, RMJ;Bannerman, DM;Rawlins, JNP
通讯作者：
Rawlins, JNP
影响因子：
1.6
作者：
CADOGAN, AK;KENDALL, DA;MARSDEN, CA
通讯作者：
MARSDEN, CA
影响因子：
2.5
作者：
Faber, ESL;Callister, RJ;Sah, P
通讯作者：
Sah, P