Does epigenetic polymorphism contribute to phenotypic variances in Jatropha curcas L.?
Does epigenetic polymorphism contribute to phenotypic variances in Jatropha curcas L.?
复制标题
DOI：
10.1186/1471-2229-10-259
复制
发表时间：
2010-11-23
影响因子：
5.3
通讯作者：
Hong Y
中科院分区：
文献类型：
--
作者：
Yi C;Zhang S;Liu X;Bui HT;Hong Y
小桐子（Jatropha curcas L.）（麻风树）作为生物柴油原料植物。其形态和种子生产力的变化已被充分记录。然而，在相同的气候和农艺措施下，不同的收藏品缺乏系统的比较评价。随着对麻风树低遗传多样性的报道，麻风树形态的遗传贡献存在不确定性。在这项研究中，从中国（CN），印度尼西亚（MD），苏里南（SU），坦桑尼亚（AF）和印度（TN）收集的麻风树植物种群种植在一个农场在相同的农艺措施。对它们的农艺性状（分枝型、株高、冠幅、始花期、休眠期、累积种子产量和含油量）进行了两年的观测和跟踪。所有的农艺性状的研究发现显着的变化。利用荧光扩增片段长度多态性（fAFLP）和甲基化敏感荧光AFLP（MfAFLP）方法对不同群体的遗传多样性和表观遗传多样性进行了分析。居群内和居群间的遗传多样性水平很低（多态性条带<0.1%）。与此相反，中间，但显着的表观遗传多样性检测（25.3%的带是多态性的）内和种群之间。MfAFLP检测到的CCGG位点中有一半以上发生甲基化，其中内胞嘧啶甲基化和双胞嘧啶甲基化在居群间差异显著。基于Nei表观遗传距离的主坐标分析（PCoA）显示坦桑尼亚/印度组与中国/印度尼西亚/苏里南组不同。表观遗传标记的遗传进行了评估，在一个F1杂交群体之间的两个形态不同的亲本植物和一个自交群体。在39个多态性标记中，有30个（77%）可遗传，符合孟德尔分离。一个表观等位基因通过其相应基因组区域的亚硫酸氢盐测序进一步确认。我们的研究证实了麻风树品种之间农艺表现的独立于气候和实践的差异。然而，这些农艺性状变异与极低的遗传多样性和中等水平但显著的表观遗传多样性相匹配。CCGG位点内胞嘧啶和双胞嘧啶甲基化在不同群体间也存在显著差异。大多数表观遗传差异标记可以作为表观等位基因遗传，遵循孟德尔分离。这些结果表明表观遗传学可能参与了麻风树的发育。
There is a growing interest in Jatropha curcas L. (jatropha) as a biodiesel feedstock plant. Variations in its morphology and seed productivity have been well documented. However, there is the lack of systematic comparative evaluation of distinct collections under same climate and agronomic practices. With the several reports on low genetic diversity in jatropha collections, there is uncertainty on genetic contribution to jatropha morphology. In this study, five populations of jatropha plants collected from China (CN), Indonesia (MD), Suriname (SU), Tanzania (AF) and India (TN) were planted in one farm under the same agronomic practices. Their agronomic traits (branching pattern, height, diameter of canopy, time to first flowering, dormancy, accumulated seed yield and oil content) were observed and tracked for two years. Significant variations were found for all the agronomic traits studied. Genetic diversity and epigenetic diversity were evaluated using florescence Amplified Fragment Length Polymorphism (fAFLP) and methylation sensitive florescence AFLP (MfAFLP) methods. Very low level of genetic diversity was detected (polymorphic band <0.1%) within and among populations. In contrast, intermediate but significant epigenetic diversity was detected (25.3% of bands were polymorphic) within and among populations. More than half of CCGG sites surveyed by MfAFLP were methylated with significant difference in inner cytosine and double cytosine methylation among populations. Principal coordinates analysis (PCoA) based on Nei's epigenetic distance showed Tanzania/India group distinct from China/Indonesia/Suriname group. Inheritance of epigenetic markers was assessed in one F1 hybrid population between two morphologically distinct parent plants and one selfed population. 30 out of 39 polymorphic markers (77%) were found heritable and followed Mendelian segregation. One epiallele was further confirmed by bisulphite sequencing of its corresponding genomic region. Our study confirmed climate and practice independent differences in agronomic performance among jatropha collections. Such agronomic trait variations, however, were matched by very low genetic diversity and medium level but significant epigenetic diversity. Significant difference in inner cytosine and double cytosine methylation at CCGG sites was also found among populations. Most epigenetic differential markers can be inherited as epialleles following Mendelian segregation. These results suggest possible involvement of epigenetics in jatropha development.
登录
查看更多内容
影响因子：
6.1
作者：
Hong, DYQ;Lau, AJ;Hong, Y
通讯作者：
Hong, Y
影响因子：
6
作者：
Kaushik, N.;Kumar, Krishan;Roy, S.
通讯作者：
Roy, S.
影响因子：
5.1
作者：
Ashikawa, I
通讯作者：
Ashikawa, I
影响因子：
1.9
作者：
Basha, S. D.;Sujatha, M.
通讯作者：
Sujatha, M.
影响因子：
3.1
作者：
Keyte, Anna L.;Percifield, Ryan;Wendel, Jonathan F.
通讯作者：
Wendel, Jonathan F.