The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation.
The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation.
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DOI：
10.1016/j.biomaterials.2011.01.029
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发表时间：
2011-05
期刊：
影响因子：
14
通讯作者：
Boyan, Barbara D.
中科院分区：
文献类型：
--
作者：
Gittens, Rolando A.;McLachlan, Taylor;Olivares-Navarrete, Rene;Cai, Ye;Berner, Simon;Tannenbaum, Rina;Schwartz, Zvi;Sandhage, Kenneth H.;Boyan, Barbara D.
钛(钛)骨结合是牙科和骨科种植体成功的关键。先前的研究表明，微米和亚微米尺度的表面粗糙度通过促进成骨细胞分化和局部因子的产生来促进骨整合。直到最近，才考虑到纳米级粗糙度对细胞响应的影响。本研究的目的是开发一种简单、可扩展的表面修饰处理方法，在不影响其他表面特征的情况下将纳米特征引入到钛基质的表面，并确定这种叠加的纳米特征对成骨细胞分化和局部因子产生的影响。开发了一种简单的氧化处理方法，在保持初始微/亚微米级粗糙度的同时，在钛表面生成可控的纳米级形貌。这种纳米改性表面也具有与原始表面相似的元素组成和接触角，但具有不同的表面晶体结构。将MG63细胞接种于机械加工(PT)、纳米改性PT(NMPT)、喷砂/酸蚀(SLA)和纳米改性SLA(NMSLA)钛圆盘上。结果表明，这种纳米结构的引入与微/亚微米级粗糙度相结合，可以促进成骨细胞的分化和局部因子的产生，这反过来又表明了改善体内种植体骨整合的潜力。
Titanium (Ti) osseointegration is critical for the success of dental and orthopaedic implants. Previous studies have shown that surface roughness at the micro- and submicro-scales promotes osseointegration by enhancing osteoblast differentiation and local factor production. Only relatively recently have the effects of nanoscale roughness on cell response been considered. The aim of the present study was to develop a simple and scalable surface modification treatment that introduces nanoscale features to the surfaces of Ti substrates without greatly affecting other surface features, and to determine the effects of such superimposed nano-features on the differentiation and local factor production of osteoblasts. A simple oxidation treatment was developed for generating controlled nanoscale topographies on Ti surfaces, while retaining the starting micro-/submicro-scale roughness. Such nano-modified surfaces also possessed similar elemental compositions, and exhibited similar contact angles, as the original surfaces, but possessed a different surface crystal structure. MG63 cells were seeded on machined (PT), nano-modified PT (NMPT), sandblasted/acid-etched (SLA), and nano-modified SLA (NMSLA) Ti disks. The results suggested that the introduction of such nanoscale structures in combination with micro-/submicro-scale roughness improves osteoblast differentiation and local factor production, which, in turn, indicates the potential for improved implant osseointegration in vivo.
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