Prevascularization of biofunctional calcium phosphate cement for dental and craniofacial repairs.
Prevascularization of biofunctional calcium phosphate cement for dental and craniofacial repairs.
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DOI：
10.1016/j.dental.2014.02.007
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发表时间：
2014-05
期刊：
影响因子：
5
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Chen, Wenchuan;Thein-Han, WahWah;Weir, Michael D.;Chen, Qianming;Xu, Hockin H. K.
关键词：
磷酸钙骨水泥(CPC)是一种很有前途的牙科和颅面修复材料。骨组织工程构建中的血管化是目前的一大挑战。本研究的目的是通过人脐静脉内皮细胞(HUVEC)和人成骨细胞(HOB)的共培养，研究大孔CPC的预血管形成，并首次确定RGD对CPC中微血管形成的影响。以CPC粉、壳聚糖液和发泡剂为主要原料，制备了大孔CPC支架。将壳聚糖与精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp，RGD)进行接枝，对CPC进行生物功能化。将人脐静脉内皮细胞和人脐静脉内皮细胞在大孔CPC-RGD和不含RGD的CPC对照组上共培养42d，观察其成骨分化、血管生成、骨基质矿物质合成和微血管样结构形成情况。与未植入RGD的CPC对照组相比，植入RGD的CPC成骨和血管形成分化标志物的基因表达增加。与CPC对照组相比，CPC-RGD组细胞合成的骨矿物质含量也增加(p<；0.05)。内皮标记物免疫组织化学染色显示，随着时间的延长，CPC支架上微血管样结构的数量增加。42d时，CPC-RGD支架的累积血管长度是CPC对照组的1.69倍。扫描电子显微镜观察证实了CPC支架上自组装的微毛细管样结构的形态。HUVEC+HOB在大孔CPC支架上共培养成功实现了预血管化。在CPC中掺入RGD可促进成骨分化、骨矿合成和微血管样结构的形成。这种新型的预血运CPC-RGD结构在牙科、颅面和矫形外科应用前景看好。
Calcium phosphate cement (CPC) is promising for dental and craniofacial repairs. Vascularization in bone tissue engineering constructs is currently a major challenge. The objectives of this study were to investigate the prevascularization of macroporous CPC via coculturing human umbilical vein endothelial cells (HUVEC) and human osteoblasts (HOB), and determine the effect of RGD in CPC on microcapillary formation for the first time. Macroporous CPC scaffold was prepared using CPC powder, chitosan liquid and gas-foaming porogen. Chitosan was grafted with Arg-Gly-Asp (RGD) to biofunctionalize the CPC. HUVEC and HOB were cocultured on macroporous CPC-RGD and CPC control without RGD for up to 42 d. The osteogenic and angiogenic differentiation, bone matrix mineral synthesis, and formation of microcapillary-like structures were measured. RGD-grafting in CPC increased the genes expressions of osteogenic and angiogenic differentiation markers than those of CPC control without RGD. Cell-synthesized bone mineral content also increased on CPC-RGD, compared to CPC control (p < 0.05). Immunostaining with endothelial marker showed that the amount of microcapillary-like structures on CPC scaffolds increased with time. At 42 d, the cumulative vessel length for CPC-RGD scaffold was 1.69-fold that of CPC control. SEM examination confirmed the morphology of self-assembled microcapillary-like structures on CPC scaffolds. HUVEC+HOB coculture on macroporous CPC scaffold successfully achieved prevascularization. RGD incorporation in CPC enhanced osteogenic differentiation, bone mineral synthesis, and microcapillary-like structure formation. The novel prevascularized CPC-RGD constructs are promising for dental, craniofacial and orthopedic applications.
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影响因子：
4.1
作者：
Chen, Wenchuan;Zhou, Hongzhi;Xu, Hockin H. K.
通讯作者：
Xu, Hockin H. K.
影响因子：
5
作者：
Cavalcanti, Bruno N.;Zeitlin, Benjamin D.;Noer, Jacques E.
通讯作者：
Noer, Jacques E.
影响因子：
3.3
作者：
Hegen, Anja;Blois, Anna;Tiron, Crina E.;Hellesoy, Monica;Micklem, David R.;Nor, Jacques E.;Akslen, Lars A.;Lorens, James B.
通讯作者：
Lorens, James B.
影响因子：
14
作者：
Hosseinkhani, Hossein;Hosseinkhani, Mohsen;Tabata, Yasuhiko
通讯作者：
Tabata, Yasuhiko
DOI：
10.1111/j.1748-1716.1972.tb05327.x
发表时间：
1972-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
ERIKSSON, E;MYRHAGE, R
通讯作者：
MYRHAGE, R