Novel circular RNA circSOBP governs amoeboid migration through the regulation of the miR-141-3p/MYPT1/p-MLC2 axis in prostate cancer.
Novel circular RNA circSOBP governs amoeboid migration through the regulation of the miR-141-3p/MYPT1/p-MLC2 axis in prostate cancer.
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新型环状RNA circSOBP通过调节前列腺癌中的miR-141- 3 p/MYPT 1/p-MLC 2轴来控制阿米巴样迁移
DOI：
10.1002/ctm2.360
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发表时间：
2021-03
影响因子：
10.6
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Chao F;Song Z;Wang S;Ma Z;Zhuo Z;Meng T;Xu G;Chen G
Metastatic prostate cancer is a fatal disease despite multiple new approvals in recent years. Recent studies revealed that circular RNAs (circRNAs) can be involved in cancer metastasis. Defining the role of circRNAs in prostate cancer metastasis and discovering therapeutic targets that block cancer metastasis is of great significance for the treatment of prostate cancer. The circSOBP levels in prostate cancer (PCa) were determined by qRT‐PCR. We evaluated the function of circSOBP using a transwell assay and nude mice lung metastasis models. Immunofluorescence assay and electron microscopic assay were applied to determine the phenotypes of prostate cancer cells’ migration. We used fluorescence in situ hybridization assay to determine the localization of RNAs. Dual luciferase and rescue assays were applied to verify the interactions between circSOBP, miR‐141‐3p, MYPT1, and phosphomyosin light chain (p‐MLC2). We observed that circSOBP level was significantly lower in PCa specimens compared with adjacent noncancerous prostate specimens, and was correlated with the grade group of PCa. Overexpression of circSOBP suppressed PCa migration and invasion in vitro and metastasis in vivo. CircSOBP depletion increased migration and invasion and induced amoeboid migration of PCa cells. Mechanistically, circSOBP bound miR‐141‐3p and regulated the MYPT1/p‐MLC2 axis. Moreover, the depletion of MYPT1 reversed the inhibitory effect of circSOBP on the migration and invasion of PCa cells. Complementary intronic Alu elements induced but were not necessary for the formation of circSOBP. The nuclear export of circSOBP was mediated by URH49. Our results suggest that circSOBP suppresses amoeboid migration of PCa cells and inhibits migration and invasion through sponging miR‐141‐3p and regulating the MYPT1/p‐MLC2 axis. CircSOBP is downregulated in prostate cancer. CircSOBP governs amoeboid migration and metastasis of prostate cancer through the miR‐141‐3p/MYPT1/p‐MLC2 axis. The nuclear export of circSOBP is mediated by URH49.
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