Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis.
Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis.
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DOI：
10.1016/j.jcv.2007.03.012
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发表时间：
2007-06
期刊：
影响因子：
--
通讯作者：
Mackay IM
中科院分区：
文献类型：
--
作者：
McErlean P;Shackelton LA;Lambert SB;Nissen MD;Sloots TP;Mackay IM
人鼻病毒（HRV）是最早发现和最常检测到的与急性呼吸道感染（ARTIs）相关的病毒，但个体血清型的分子流行病学和基因组变异仍不确定。对一种新的HRV进行分子标记，并确定其在主要儿科人群中的患病率和临床影响。核苷酸测序用于确定完整的HRV-QPM编码序列。设计了两种新型实时RT-PCR诊断检测方法，并用于回顾性筛选1244例样本提取物的明确人群，以确定2003年期间HRV-QPM的患病率。完整编码序列的系统发育研究将HRV-QPM定义为鼻病毒属的新成员，其位于先前描述的HRV-A2亚谱系内。对相对较短的VP 1序列的研究表明，该病毒对Pleconaril具有耐药性，将其与HRV A种区分开来。16个额外的HRV-QPM菌株被检测到（1.4%的标本），通常作为唯一的微生物存在于疑似毛细支气管炎的婴儿。2006年在欧洲也发现了HRV-QPM，2004年在美国也发现了一种密切相关的病毒。我们提出的分子特征和初步的临床影响，一个新发现的HRV沿着与序列代表额外的新的HRV。
Human rhinoviruses (HRVs) are some of the earliest identified and most commonly detected viruses associated with acute respiratory tract infections (ARTIs) and yet the molecular epidemiology and genomic variation of individual serotypes remains undefined. To molecularly characterise a novel HRV and determine its prevalence and clinical impact on a predominantly paediatric population. Nucleotide sequencing was employed to determine the complete HRV-QPM coding sequence. Two novel real-time RT-PCR diagnostic assays were designed and employed to retrospectively screen a well-defined population of 1244 specimen extracts to identify the prevalence of HRV-QPM during 2003. Phylogenetic studies of complete coding sequences defined HRV-QPM as a novel member the genus Rhinovirus residing within the previously described HRV-A2 sub-lineage. Investigation of the relatively short VP1 sequence suggest that the virus is resistant to Pleconaril, setting it apart from the HRV A species. Sixteen additional HRV-QPM strains were detected (1.4% of specimens) often as the sole micro-organism present among infants with suspected bronchiolitis. HRV-QPM was also detected in Europe during 2006, and a closely related virus circulated in the United States during 2004. We present the molecular characterisation and preliminary clinical impact of a newly identified HRV along with sequences representing additional new HRVs.
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影响因子：
82.9
作者：
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通讯作者：
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