Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells.
Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells.
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DOI：
10.1111/jcmm.12529
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发表时间：
2015-02
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Cismaşiu VB;Popescu LM
端细胞（Telocytes，TC）是广泛分布于人体内的一类细胞，其特征是具有细长的端足细胞（telopodes，Tps）。心脏TC是目前最好的特征性TC。Tps在体内和体外释放细胞外囊泡（EV），表明TC通过囊泡旁分泌信号调节其他细胞的活性。TC已经在几个器官的干细胞龛中被发现。电子显微镜或电子断层扫描显示，Tps位于干细胞（SC）附近。由于干细胞调控的利基组件涉及旁分泌信号，我们已经调查，如果TC可能是这种机制的一部分。使用钙黄绿素和Cy 5-miR-21寡核苷酸对细胞和EV进行荧光标记，我们提供了TC可以通过装载有microRNA的EV调节SC的证据。TC将微小RNA递送至心脏干细胞（CSC）以及其他类型的SC（例如造血SC），表明该机制不限于心脏组织。我们还发现，CSC提供microRNA加载EV的TC，这表明有一个连续的，转录后调控信号来回之间的TC和SC。总之，我们的数据揭示了存在一个相互的（双向）表观遗传信号之间的TC和SC。
Telocytes (TCs) are cells ubiquitously distributed in the body and characterized by very long and thin prolongations named telopodes (Tps). Cardiac TCs are the best characterized TCs for the moment. Tps release extracellular vesicles (EVs) in vivo and in vitro suggesting that TCs regulate the activity of other cells by vesicular paracrine signals. TCs have been found within the stem cell niche of several organs. Electron microscopy or electron tomography has shown that Tps are located in close vicinity of stem cells (SC). Since stem cell regulation by niche components involves paracrine signalling, we have investigated if TCs could be part of this mechanism. Using fluorescent labelling of cells and EVs with calcein and Cy5-miR-21 oligos, we provide evidence that TCs can modulate SC through EVs loaded with microRNAs. TCs deliver microRNA to cardiac stem cells (CSCs), as well as to other types of SCs (e.g. hematopoietic SC) indicating that this mechanism is not restricted to cardiac tissue. We also found that CSCs deliver microRNA loaded EVs to TCs, suggesting that there is a continuous, post-transcriptional regulatory signal back and forth between TCs and SC. In conclusion, our data reveal the existence of a reciprocal (bidirectional) epigenetic signalling between TCs and SC.
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影响因子：
5.3
作者：
Luesma MJ;Gherghiceanu M;Popescu LM
通讯作者：
Popescu LM
影响因子：
5.3
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
通讯作者：
Simionescu O
影响因子：
3.7
作者：
Collino F;Deregibus MC;Bruno S;Sterpone L;Aghemo G;Viltono L;Tetta C;Camussi G
通讯作者：
Camussi G
影响因子：
5.3
作者：
Manole CG;Cismaşiu V;Gherghiceanu M;Popescu LM
通讯作者：
Popescu LM
影响因子：
--
作者：
Cretoiu, Sanda M;Popescu, Laurentiu M
通讯作者：
Popescu, Laurentiu M