Synergistic Effect of Mesoporous Silica and Hydroxyapatite in Loaded Poly(DL-lactic-co-glycolic acid) Microspheres on the Regeneration of Bone Defects.
Synergistic Effect of Mesoporous Silica and Hydroxyapatite in Loaded Poly(DL-lactic-co-glycolic acid) Microspheres on the Regeneration of Bone Defects.
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负载聚DL-乳酸-乙醇酸共聚物微球中介孔二氧化硅和羟基磷灰石对骨缺损再生的协同作用
DOI：
10.1155/2016/9824827
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发表时间：
2016
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
He S;Lin KF;Fan JJ;Hu G;Dong X;Zhao YN;Song Y;Guo ZS;Bi L;Liu J
制备了聚乳酸-羟基乙酸共聚物（PLGA）、介孔二氧化硅纳米粒子（MSN）和纳米羟基磷灰石（nHA）复合微球（PLGA-MSN/nHA），并对其作为骨组织工程材料的性能进行了评价。本研究的目的是研究MSN/nHA对生物相容性的协同作用以及其潜在的骨形成能力。首先，我们发现这种PLGA-MSN/nHA复合材料在微观结构、机械强度和润湿性方面表现出良好的特性。通过细胞培养实验，PLGA-MSN/nHA复合材料上种植的细胞的粘附和增殖率高于对照组，逆转录聚合酶链反应检测到高水平的成骨因子如ALP和Runx-2。最后，将该PLGA-MSN/nHA复合材料植入兔股骨骨缺损模型中，通过组织学检查观察其诱导骨再生的能力。植入12周后，骨缺损处的成熟骨形成明显多于对照组，残留材料明显少于对照组。实验结果表明，将MSN和nHA引入PLGA微球中制备的PLGA-MSN/nHA复合材料在体内外均能提高复合材料的生物相容性和成骨诱导性，在骨再生方面具有潜在的应用价值。
A microsphere composite made of poly(DL-lactic-co-glycolic acid) (PLGA), mesoporous silica nanoparticle (MSN), and nanohydroxyapatite (nHA) (PLGA-MSN/nHA) was prepared and evaluated as bone tissue engineering materials. The objective of this study was to investigate the synergistic effect of MSN/nHA on biocompatibility as well as its potential ability for bone formation. First, we found that this PLGA-MSN/nHA composite performed good characteristics on microstructure, mechanical strength, and wettability. By cell culture experiments, the adhesion and proliferation rate of the cells seeded on PLGA-MSN/nHA composite was higher than those of the controls and high levels of osteogenetic factors such as ALP and Runx-2 were detected by reverse transcriptase polymerase chain reaction. Finally, this PLGA-MSN/nHA composite was implanted into the femur bone defect in a rabbit model, and its ability to induce bone regeneration was observed by histological examinations. Twelve weeks after implantation, the bone defects had significantly more formation of mature bone and less residual materials than in the controls. These results demonstrate that this PLGA-MSN/nHA composite, introducing both MSN and nHA into PLGA microspheres, can improve the biocompatibility and osteoinductivity of composite in vitro and in vivo and had potential application in bone regeneration.
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影响因子：
16.1
作者：
Agarwal R;García AJ
通讯作者：
García AJ
影响因子：
--
作者：
Hui, JHP;Li, L;Lee, EH
通讯作者：
Lee, EH
影响因子：
2.9
作者：
Chun, KW;Yoo, HS;Park, TG
通讯作者：
Park, TG
影响因子：
4.9
作者：
Kohane, DS;Tse, JY;Langer, R
通讯作者：
Langer, R
影响因子：
3.4
作者：
Liu, Hongfei;Shi, Shuangshuang;Xi, Jumei
通讯作者：
Xi, Jumei