Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials.
Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials.
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DOI：
10.1007/s10103-018-2524-7
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发表时间：
2018-09
影响因子：
2.1
通讯作者：
Kang L
中科院分区：
文献类型：
--
作者：
Abdulrehman G;Xv K;Li Y;Kang L
本研究旨在探讨间四羟基苯基二氢卟酚介导的光动力疗法（m-THPC-PDT）对人结肠癌细胞株SW 480和转移性结肠癌细胞株SW 620的抗肿瘤作用及其可能机制。将SW 480和SW 620细胞与各种浓度的m-THPC孵育，然后进行光动力照射。用激光共聚焦显微镜观察m-THPC在细胞内的亚细胞定位。MTT法检测m-THPC对两种细胞的光毒性。流式细胞仪检测细胞凋亡。通过划痕试验和集落形成试验分别测定其迁移能力和长期恢复能力。CLSM结果显示，m-THPC主要分布于SW 480细胞的内质网和溶酶体中，SW 620细胞的溶酶体和线粒体中。m-THPC-PDT对SW 480和SW 620细胞的杀伤作用呈剂量依赖性和光能依赖性。当m-THPC浓度增加到11.76 μM时，SW 480和SW 620的凋亡率分别约为65%和25%。而两种细胞系的坏死细胞率无明显变化。m-THPC-PDT处理后两种细胞系殖民地形成和迁移能力均呈剂量依赖性下降。m-THPC光动力疗法不仅能有效抑制细胞增殖，降低细胞的迁移能力和集落形成能力，而且能有效杀伤SW 480和SW 620细胞，并呈剂量依赖性。这些结果表明，m-THPC是一种有前途的致敏剂，值得进一步开发和广泛的研究，以临床使用的结直肠癌。
The aim of this study is to investigate the antitumor effects and possible mechanisms of meta-tetrahydroxyphenylchlorin-mediated photodynamic therapy (m-THPC-PDT) on human primary (SW480) and metastatic (SW620) colon cancer cell lines. SW480 and SW620 cells were incubated with various concentrations of m-THPC, followed by photodynamic irradiation. Subcellular localization of m-THPC in cells was observed with confocal laser scanning microscopy (CLSM). Photocytotoxicity of m-THPC in the two cells was investigated by using MTT assay. The flow cytometry was employed to detect the cell apoptosis. The migration and long-term recovery ability were determined by scratch test and colony formation assay respectively. CLSM showed that m-THPC was mainly distributed within the endoplasmic reticulum (ER) and lysosome of SW480 cells and within the lysosome and mitochondria of SW620 cells. m-THPC-PDT induced a dose-dependent and light energy-dependent cytotoxicity in SW480 and SW620 cells. Apoptosis rate was approximately 65 and 25% in SW480 and SW620 respectively when the concentration of m-THPC increased to 11.76 μM. However, the rate of necrotic cells had no significant changes in two cell lines. The colony formation and migration ability of the two cell lines were decreased with m-THPC-PDT treatment in a dose-dependent manner. PDT with m-THPC not only could effectively inhibit cell proliferation and decrease migration ability and colony formation ability, but also could effectively kill SW480 and SW620 cells in a dose-dependent manner in vitro. These results suggest that m-THPC is a promising sensitizer that warrants further development and extensive studies towards clinical use of colorectal cancer.
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DOI：
10.3322/caac.20114
发表时间：
2011-07
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Agostinis P;Berg K;Cengel KA;Foster TH;Girotti AW;Gollnick SO;Hahn SM;Hamblin MR;Juzeniene A;Kessel D;Korbelik M;Moan J;Mroz P;Nowis D;Piette J;Wilson BC;Golab J
通讯作者：
Golab J
影响因子：
3.2
作者：
Benizri, Emmanuel I.;Bernard, Jean-Louis;Bereder, Jean-Marc
通讯作者：
Bereder, Jean-Marc
影响因子：
3.3
作者：
Kawczyk-Krupka, Aleksandra;Latos, Wojciech;Sieron, Aleksander
通讯作者：
Sieron, Aleksander
影响因子：
--
作者：
Kohl, E.;Karrer, S.
通讯作者：
Karrer, S.
影响因子：
2.6
作者：
Durbec, M.;Cosmidis, A.;Ceruse, P.
通讯作者：
Ceruse, P.