Relationship between Gingival Crevicular Fluid Microbiota and Cytokine Profile in Periodontal Host Homeostasis.
Relationship between Gingival Crevicular Fluid Microbiota and Cytokine Profile in Periodontal Host Homeostasis.
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龈沟液微生物群与牙周宿主稳态中细胞因子谱的关系
DOI：
10.3389/fmicb.2017.02144
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发表时间：
2017
影响因子：
5.2
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Zhou J;Yao Y;Jiao K;Zhang J;Zheng X;Wu F;Hu X;Li J;Yu Z;Zhang G;Jiang N;Li Z
作为牙周炎潜在的生物标志物，微生物组和细胞因子最近得到了广泛的研究，但对微生物结构和细胞因子组成之间的变化进行联合分析的情况很少。本研究旨在调查患有牙周炎和不患有牙周炎的个体在微生物群和细胞因子的组合图谱上是否存在差异。用454型焦磷酸测序和RayBio双抗体芯片分析了鸡石山(甘肃，中国)16例牙周炎患者和15例正常对照牙周沟液中微生物菌群和细胞因子的组成。结果表明，与健康组相比，牙周炎组与健康组相比，属的同现率更高，这是由肖纳基于丰度的同现指数来评价的。C反应蛋白(CRP)在牙周炎组中显著升高(P<0.05)，而白细胞介素8(IL)-8在健康组中显著升高(P<；0.01)。MANTEL试验显示细胞因子与微生物区系有显著的一致性，在健康组(MANTEL统计量r=0.36，P≤0.05)，而在牙周炎组(MANTEL统计量r=0.013，P=0.434)。这一结果得到了普罗克斯特试验的进一步证实。在门、纲、目、科和属水平上，基质金属蛋白酶(MMP)-9、骨活素、IL-8和巨噬细胞炎性蛋白(MIP)-1a与细菌组成显著相关。在物种水平上，C反应蛋白也与细菌组成有关。总而言之，多菌群落结构的变化导致了细胞因子和微生物群之间的健康相关性的中断。这种微生物区系和免疫反应之间的失衡可能是牙周炎的主要病因机制之一。
As potential biomarkers in periodontitis, microbiome, and cytokines have recently been extensively investigated, but combined analyses of the variations between the microbial structure and cytokine composition are rare. The present study aimed to investigate whether there are differences in the combined profile of microbiome and cytokines in individuals with or without periodontitis. The microbiome and cytokine composition in gingival crevicular fluid (GCF) from 16 patients and 15 controls from Jishi Shan (Gansu, China) were analyzed using 454 pyrosequencing and RayBio Quantibody Arrays. The results showed that a higher co-occurrence of genera in periodontitis group compared with the healthy group, as evaluated by Schoener's abundance-based co-occurrence index. C-reactive protein (CRP) was significantly (P < 0.05) higher in the GCF of the periodontitis group while interleukin (IL)-8 was significantly (P < 0.01) higher in the GCF of the healthy group. The Mantel test revealed a significant concordance between cytokines and microbiota, in the healthy group (Mantel statistic r = 0.36, P ≤ 0.05) but not in the periodontitis group (Mantel statistic r = 0.013, P = 0.434). The results were further confirmed by the Procrustes test. Matrix metalloproteinase (MMP)-9, osteoactivin, IL-8, and macrophage inflammatory protein (MIP)-1a were significantly associated with bacterial composition at the phylum, class, order, family, and genus levels. CRP was also associated with bacterial composition at the species level. In conclusion, alterations in the polymicrobial community structure leads to disruption in the healthy correlation between cytokines and microbiomes. This dysbiosis between the microbiota and the immune response could be one of the major etiological mechanisms underlying periodontitis.
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影响因子：
--
作者：
Gursoy, Ulvi Kahraman;Kononen, Eija;Sorsa, Timo
通讯作者：
Sorsa, Timo
对口腔癌检测的唾液生物标志物的研究综述。
影响因子：
10.6
作者：
Cheng YS;Rees T;Wright J
通讯作者：
Wright J
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
2.7
作者：
Crielaard W;Zaura E;Schuller AA;Huse SM;Montijn RC;Keijser BJ
通讯作者：
Keijser BJ
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.