Affinity Separations Using Foam Fractionation

研究重点是测试泡沫分馏新概念的可行性，该概念利用水溶液中的蛋白质与烃类链上的抑制剂、辅因子和抗原的亲和结合。研究的具体目的是：琥珀酸脱氢酶（SDH）抑制剂的结合亲和力将会改变，以确定其对泡沫分馏器表面活性的影响。两种不同的酶SDH和Carboxpeptidase A （CPA）将结合到两种具有相当结合常数的抑制剂上，以确定酶结构对表面活性的影响。不同的烷基尾长度对改性抑制剂酶复合物表面活性的影响将被评估。

Research focuses on testing the feasibiity of a new concept of foam fractionation using affinity binding of proteins in aqueous solution to inhibitors, cofactors, and antigens attached to hydrocarbon chains. Specfic aims of the research are: the binding affinity of an inhibitor for succinic dehydrogenase (SDH) will be varied to determine its effect on surface activity in a foam fractionator. Two different enzymes, SDH and Carboxpeptidase A (CPA), will be bound to two inhibitors of comparable binding constant to determine the effect of enzyme structure on surface activity. The effect of varying alkyl tail length on the surface activity of a modified inhibitor enzyme complex will be assessed.