High Performance Liquid Chromatography in Undergraduate Chemistry Laboratories

该项目的目的是通过将高效液相色谱(HPLC)纳入本科实验室课程来改进蒙茅斯学院的化学教学，以补充课堂上教授的分离理论。学生将在定量分析、仪器分析和有机化学实验课程中使用高效液相仪器，以获得对这种强大的现代化合物分离和分析方法的直接体验和欣赏。学生将进行“真实”样品混合物的分析；确定洗脱液成分、柱温和梯度洗脱的影响；并监测有机反应的进展。在高效液相色谱的理论、操作和应用方面获得的宝贵经验将有助于学生日后在工作场所和研究生研究中取得成功。

The aim of this project is to improve chemistry instruction at Monmouth College by incorporating high performance liquid chromatography (HPLC) into the undergraduate laboratory curriculum, to complement separation theory taught in lecture courses. The HPLC instrumentation will be used by students in quantitative analysis, instrumental analysis, and organic chemistry laboratory courses, to gain direct experience with and apprecia-tion for this powerful modern method of separation and analysis of compounds. Students will conduct analyses of "real-life" sample mixtures; determine effects of eluent composition, column temperature, and gradient elution; and monitor progress of organic reactions. The valuable experie-nce gained with the theory, operation, and applications of HPLC will contribute to students' later success in the workplace and in graduate research.