High Performance Liquid Chromatography (HPLC) for Under- graduate Laboratories

该项目将为密歇根大学迪尔伯恩分校的本科生提供现代高效液相仪器所提供的实验能力。这些设备将在整个课程中使用，包括有机、高级有机、高级无机、分析和环境化学实验室、生物化学、分子生物学，以及一门关于色谱分离的特殊课程。本科生将进行实验，展示高效液相色谱法的多学科应用，以及这项功能强大、应用广泛的实验技术的各种分析应用，从化合物鉴定和产品百分比分配到分离系统的设计和优化。学生将获得折射率和紫外线/可见光吸收度检测器的经验，以及高效液相仪器的微处理器控制。

This project will provide undergraduate students at the University of Michigan-Dearborn with experimental capabilities afforded by modern HPLC instrumentation. The equipment will be used in courses throughout the curriculum, including organic, advanced organic, advanced inorganic, analytical, and environmental chemistry laboratories, biochemistry, molecular biology, and a special course in chromatographic separations. Undergraduates will perform experiments which illustrate the multidisciplinary uses of HPLC and the variety of analytical applications of this powerful, widely used experimental technique, from compound identification and percent product distribution to design and optimization of separation systems. The students will gain experience with both refractive index and ultraviolet/visible absorbance detectors, and with microprocessor control of HPLC instrumentation.