Fundamental Studies of Aqueous Polymeric Two-Phase Systems for Bioseparations

现代生物技术产品的质量常常受到分离操作效果的限制。分离技术的有效性对产品的生物活性、纯度和安全性至关重要。由于生物材料必须在其自然环境中进行浓缩和分离，因此必须开发特殊的加工和处理技术。这个程序解决了一个这样的需求，并着重于一个多相萃取系统，该系统由两个水相组成，其中感兴趣的蛋白质被轻轻地处理而不变性。这项研究将探索如何将蛋白质分离成两相中的一种，并在重力的影响下通过沉淀来纯化。因此，在分离步骤中不需要特殊的机器。

The quality of the products of modern biotechnology often are limited by the effectiveness of the separation operations. The effectiveness of the separations technology is central to the biological activity, purity and safety of the products. Since biological materials must be concentrated and separated in their natural environment, special techniques must be developed for their processing and treatment. This program addresses one such need and focuses on a multiphase extraction system consisting of two aqueous (water) phases in which the proteins of interest are handled gently without denaturation. The research will explore how the protein can be segregated into one of the two phases and could be purified by settling under the influence of gravity. Thus no special machinery would be needed in the separation step.