Research Initiation Award: Investigation of the Effects of Dissolved Oxygen and Dissolved Carbon Dioxide on Embryogenesis and the Competence for Embryogenesis

叙述：胡萝卜的体细胞胚胎发生将被用作 模型来量化溶解氧和溶解 二氧化碳对未分化细胞生长和胚胎发生的影响。 选择此模型是因为有关 胡萝卜的体细胞胚胎发生已经存在于文献中。 胚胎被认为起源于单细胞，或一个小的 与小区集群相关联的小区数量。 后 诱导，胚胎通过几个阶段的进展， 发展 对胚胎发育各个阶段的认识 和植株再生将包括主要的分析 评估该项目胚胎发生的程序。 各种组成的气体将用于摇瓶研究， 这些信息将被转移到更工业化的 搅拌的、可扩展的反应器的适用系统。 氧气和 将研究二氧化碳的影响，以开发一种生物反应器， 可以在受控的气体组分水平下操作。 这一发展将提供一个宝贵的工具， 研究将需要培养胚胎， 明确的营养条件。

Narrative: The somatic embryogenesis of carrots will be used as a model to quantify the effects of dissolved oxygen and dissolved carbon dioxide on undifferentiated cell growth and embryogenesis. This model has been selected because extensive information regarding somatic embryogenesis of carrots already exists in the literature. Embryos are thought to originate from single cells, or a small number of cells associated with a cluster of cells. After induction, the embryos progress through several stages of development. Recognition of the various stages of embryogenesis and plantlet regeneration will comprise the major analytical procedures for assessing embryogenesis for this project. Gases of various composition will be used in shake flask studies and the information will then be transferred to the more industrially applicable system of an agitated, scalable reactor. The oxygen and carbon dioxide effects will be studied to develop a bioreactor that can be operated at controlled levels of the gaseous components. This development will provide a valuable tool for further investigations that will require the cultivation of embryos under well defined nutritional conditions.