Techniques of Flow Cytoenzymological Analysis

该提案要求资金用于开发新的流动 细胞计数仪器和操作程序 大型真核细胞的细胞酶学分析， 直径在20- 120 μ m范围内的细胞。 研究 专注于开发用于流动的新型基质， 细胞酶学和技术的发展，使 基于表面积对细胞进行实时分析和分选， 体积和浓度。 这些技术将用于 分析了影响表达的因素， 适当工程化的嵌合基因的植物，特别是β- 葡萄糖醛酸酶，从细菌转移后， 活植物原生质体流式分选 根据导入基因的表达水平，

This proposal requests funds for the development of novel flow cytometric instrumentation and operating procedures for use in the cytoenzymological analysis of large eukaryotic cells, those cells having diameters in the range of 20-l20 um. The research focuses on the development of novel substrates for use in flow cytoenzymology and on the development of techniques to permit the real-time analysis and sorting of cells based on surface area, volume, and concentration. The techniques will be employed for the analysis of those factors involved in the expression in plants of suitably engineered chimaeric genes, specifically beta- glucuronidase, following transfer from bacteria, and for the selection through flow sorting of living plant protoplasts according to the level of expression of the introduced genes.