U.S.-Korea Cooperative Research on DNA Sequence Effects on Open Complex Formation by RNA Polymerase

这一建议要求资金允许M博士。托马斯记录，部门 威斯康星州大学化学系，与Jung-Hye Roe博士合作， 首尔国立大学微生物学系，为期 24个月，一项关于DNA序列对 通过RNA聚合酶形成开放复合物。 序列效应 转录起始位点附近的变异对形成速率的影响 并将研究开放复合物的解离。 三步走 开放复合体形成的机制，由合作者在 以前的研究，将被检查，试图确定 中间步骤。 特别是，序列变异的影响 在不同溶液条件下的结合和解离速率 条件将进行研究。 在原核生物中，基因表达的程度和时间是 主要在转录起始水平上调节。 转录调控需要特定蛋白质的相互作用 DNA上的特定位点。 在目前的研究中， 诱变E. coli DNA将被用来研究 _ ____ 引入变体启动子对形成速率的影响， RNA聚合酶开放复合物在不同条件下的解离 温度、阳离子和阴离子浓度等条件。 雷德博士和罗伊博士都是德高望重的科学家， 在此之前，他们曾在这一领域进行过成功的合作。 这 该项目与美国的目标有关-韩国合作社 旨在提高合作水平的科学计划 美国和韩国的科学家和工程师通过交流， 科学信息、思想、技能和技术， 在互惠互利的问题上进行合作。

This proposal requests funds to permit Dr. M. Thomas Record, Department of Chemistry, University of Wisconsin, to pursue with Dr. Jung-Hye Roe, Department of Microbiology, Seoul National University, for a period of 24 months, a program of cooperative research on DNA sequence effects on open complex formation by RNA polymerase. The effect of sequence variations near the transcriptional start site on the rate of formation and dissociation of the open complex will be studied. The three-step mechanism of open-complex formation, proposed by the collaborators in previous research, will be examined in an attempt to identify intermediate steps. In particular, the effects of sequence variations on the rates of association and dissociation under various solution conditions will be studied. In prokaryotes the extent and the timing of gene expression is regulated primarily at the level of transcription initiation. Transcriptional control requires the interaction of specific proteins with specific sites on DNA. In the present research, site-specific mutagenesis of E. coli DNA will be employed to study the effect of the _ ____ introduction of variant promoters on the rates of formation and dissociation of open complexes of RNA polymerase under different conditions of temperature, concentrations of cations and anions, etc. Drs. Record and Roe are highly respected scientists who have successfully collaborated previously in this field of research. This project is relevant to the objectives of the U.S.-Korea Cooperative Science Program which seeks to increase the level of cooperation between U.S. and Korean scientists and engineers through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit.