MRI: Development of Peptidergic Cells in the SympathoadrenalSystem

周围神经系统的形成，几乎全部是由神经嵴细胞在发育中的胚胎的特定区域迁移和结合而形成的。在神经节发生的过程中，这些细胞获得了特定的性状，并最终分化成成人的各种表型。本研究的目的是：(1)记录周围神经系统交感肾上腺支多肽能表型的发展；从成年鸡和鹌鹑的神经嵴细胞到终末分化状态；(2)通过在条件培养基、无细胞提取物或激素的存在下培养神经嵴细胞和分离的胚胎神经节细胞，研究外部因素对多肽能表达的影响；以及在发育冠细胞时可能遇到的其他因素。该项目的结果将提供发育中的周围神经系统中肽能细胞群及其出现的时间过程的精确地图。他们还将定义环境机制对诱导和维持肽能表型的贡献。此外，这些结果将提供基本的胚胎学信息，可以极大地帮助我们理解周围神经系统发育和可塑性的机制。

The peripheral nervous system is formed, almost in its entirety by neural crest cells that migrate and associate in specific areas of the developing embryo. During the process of gangliogenesis these cells acquire particular traits and eventually differentiate into the various phenotypes found in the adult. The aims of this research are: (1) to document the development of peptidergic phenotypes in the sympathoadrenal branch of the peripheral nervous system, from neural crest cells to the terminally differentiated state found in the chicken and quail adult and (2) to examine the influence of extrinsic factors on peptidergic expression by culturing neural crest cells and dissociated embryonic ganglion cells in the presence of conditioned media, cell free extracts, or hormones, and other factors likely to be encountered by developing crest cells. Results from this project will provide a precise map of the peptidergic cell populations in the developing peripheral nervous system and the time course of their appearance. They will also define the contribution of environmental mechanisms to the induction and maintenance of peptidergic phenotypes. In addition, these results will provide basic embryological information that could aid considerably our understanding of the mechanisms underlying the development and plasticity of peripheral nervous system.