A Model System for Analyzing the Generation of Linear Satellite RNAs

小的感染性RNA，如卫星和类病毒， 干扰基本但尚未鉴定的分子 植物细胞中的过程。 许多注意力都集中 最近关于圆形小的成熟形式的生成 感染性RNA。 发现一些卫星和 类病毒进行自我裂解反应，而其他类病毒仅 在核提取物存在的过程中， 对RNA多功能特性的基本认识 分子。 为了研究线性卫星RNA的产生， 芜菁皱缩病毒卫星系统是理想的， 多个相关的卫星，体外的感染性 改变的卫星和最近确定的一个区域 正常单体卫星积累所必需的。 采用 体外改变卫星序列，然后在 感染性cRNA的体外转录和接种 植物，参与卫星处理的核苷酸， 这个区域以及其他区域将被精确地识别。 到 更好地理解这种潜在的新颖处理机制， 处理中涉及的序列将被复制、删除 并移动到卫星分子的不同位置。 到 在数据分析的帮助下，体外处理系统将 利用受感染和未受感染植物的提取物研制而成。 小致病RNA的研究是一个特别重要的领域， 植物生物学的挑战和有趣的领域。 即使 这些RNA可以用分子术语来描述，它们的表达模式 行动仍然没有解决。 事实上，他们可以有 对植物发育的显著影响表明， 简单的致病RNA必须以某种方式相互作用并干扰 正常植物细胞中的基本分子机制。

Small infectious RNAs, such as satellites and viroids, can interfere with fundamental but as yet unidentified molecular processes in plant cells. Much attention has been focused recently on the generation of mature forms of circular small infectious RNAs. The discovery that some satellites and viroids undergo self-cleavage reactions while others only process in the presence of nuclear extracts is increasing our basic understanding of the multifunctional properties of RNA molecules. To study the generation of linear satellite RNAs, the turnip crinkle virus satellite system is ideal because of the multiple related satellites, the infectivity of in vitro altered satellite and the recent identification of a region essential for normal monomeric satellite accumulation. By in vitro alteration of the satellite sequence, followed by in vitro transcription of infectious cRNA and inoculation in plants, the nucleotides involved in satellite processing in this region as well as others will be precisely identified. To better understand this potentially novel processing mechanism, sequences involved in processing will be duplicated, deleted and moved to different locations in the satellite molecule. To aid in data analysis, an in vitro processing system will be developed using extracts from infected and uninfected plants. The study of small pathogenic RNAs is a particularly challenging and intriguing area of plant biology. Even though these RNAs can be described in molecular terms, their mode of action remains unsolved. The fact that they can have significant effects on plant development suggests that these simple pathogenic RNAs must somehow interact and interfere with fundamental molecular mechanisms in the normal plant cell.