Research Initiation: Thin-Gel Composite Membranes for the Separation of Biological Molecules

本研究的目标是开发薄凝胶复合膜 用于生物分离的凝胶色谱。 的 所提出的复合膜将通过界面 聚合到薄的多孔载体上。 使用这种薄凝胶 复合膜可导致大规模的生物分离。 的 生产率、选择性和有效厚度 膜将与聚合条件相关。 水- 代表生物分子大小的可溶性聚合物将 用染料分子标记并用作渗透溶质。 膜通量和选择性将通过流通测定 扩散池使用光纤探针色度计， 即时测量下游浓度。 这个项目 将刺激凝胶基膜领域的进一步研究 分居 基于薄凝胶复合膜的工艺 潜在地可以替代纯化中的额外步骤， 蛋白质和其他生物分子。

The goal of the research is to develop thin gel composite membranes for application in gel chromatography for bioseparations. The proposed composite membranes will be formed by interfacially polymerizing onto thin porous supports. Use of such thin gel composite membranes could result in large-scale bioseparations. The productivity, selectivity, and effective thickness of the resulting membranes will be related to the polymerization conditions. Water- soluble polymers that are representative of biomolecular size will be tagged with dye molecules and used as the permeating solutes. Membrane flux and selectivity will be determined with a flow-through diffusion cell which uses a fiber optic probe colorimeter to instantaneously measure the downstream concentration. This project will stimulate further research in the area of gel-based membrane separations. Processes based on the thin-gel composite membrane potentially may replace additional steps in the purification of proteins and other biological molecules.