Developing Polymeric Separating Agents to Mimic the Specificity of Protein Binding

这项拟议的工作是朝着开发模拟生物结合机制的合成聚合物吸附剂迈出的第一步。在特定位置表现出多个相互作用机制和竞争的蛋白质，选择性地满足那些与特定结合点能量最接近的蛋白质材料。这项研究最初将展示一种能够选择性吸收溶质的分离剂，这种分离剂需要同时利用氢键和疏水相互作用的机制。要通过模拟自然来开发改进的分离剂，需要对相关的机械相互作用有彻底的了解。虽然关于蛋白质如何与分子结合产生了大量的信息，但关于固-液吸附机制的知识却相当少。因此，建议初步研究三种结合机制：氢键、疏水相互作用和电荷转移络合作用。最后，在这项工作的后期阶段，这些相互作用将被耦合起来，以开发模拟生物结合的分离剂。为了更好地理解这些现象，并允许对这些结果进行外推，已经开发了适当的热力学描述。

The proposed work is an initial step toward developing synthetic polymeric sorbents that mimic biological binding mechanisms. Proteins exhibiting multi-interactive mechanisms and competition at a particular site are selectively satisfied by those protein materials which most closely fit energetically a specific binding point. The research will initially demonstrate a separating agent capable of selectively absorbing solutes that require mechanisms which utilize both hydrogen bonding and hydrophobic interaction. To develop improved separating agents by mimicing nature requires a thorough understanding of relevant mechanistic interactions. Although a large amount of information is being generated on how proteins bind to molecules, there is considerably less knowledge concerning solid-liquid adsorption mechanisms. Thus, it is proposed to initially examine three binding mechanisms; hydrogen bonding, hydrophobic interactions and charge transfer complexation. Finally, in the later stages of the work it follows that these interactions will be coupled to develop separating agents which mimic biological binding. To provide a better understanding of these phenomena as well as to permit the extrapolation of these results, appropriate thermodynamic descriptions have been developed.