Cellular Characterization of the Maize Auxin Receptor

生长是所有细胞的基本过程，由一种特定的 激素与其受体在特定细胞内的相互作用 地址. 在植物中，生长是由以下物质的特异性相互作用介导的： 然而，生长促进激素，生长素和生长素受体， 现在有大量的证据，尽管仍然是间接的， 生长素受体及其基因已被鉴定。 有几 这种受体的独特功能，最重要的是，它的大部分 被发现隐藏在一个细胞区室中 无其他生长 激素受体也具有这种特性，这就提出了一个问题， 这种受体感知外部激素的机制 并调节生长。 本提案涉及以下问题： 组织和细胞是生长素受体的所在地， 细胞发育过程中的变化？ 生长素的细胞部位在哪里 生长素受体的感知，这是不同的， 大部分受体的位置 是否存在 受体与生长素诱导生长能力相关？ 在许多情况下，植物的生长受限于植物中的微生物的丰度。 生长素受体 了解这种限制是如何规定的 将使我们了解人类如何操纵植物 生长和作物产量。

Growth, a fundamental process of all cells, is mediated by a specific interaction between a hormone and its receptor at a specific cellular address. In plants, growth is mediated by the specific interaction of the growth-promoting hormone, auxin, and the auxin receptor, however, there is now substantial evidence, although it is still indirect, that the auxin receptor and its gene has been identified. There are several unique features of this receptor, the most important is that most of it is found sequestered in a cellular compartment. No other growth hormone receptor shares this properties and it raises the question of the mechanism by which this receptor perceives hormone on the outside of the cell and mediates growth. The following questions are addressed in this proposal: Where in the tissue and cell is the auxin receptor located and does this location change during cell development? Where is the cellular site of auxin perception by the auxin receptor and is this different than the location of the bulk of the receptor population? Does the presence of the receptor correlate with auxin-induced growth capacity? In many instances, growth in plants is limited by the abundance of the auxin receptor. An understanding of how this limitation is regulated in the plant will lend insight into how mankind can manipulate plant growth and crop yields.