Site-Directed Bioreagent Immobilization for Development of Microbiosensor Arrays

该项目将尝试合并微电子，化学- 选择性膜和生物试剂（酶和抗体）， 生产集成的生物传感器阵列。 它将利用最近的 在这三个方面取得了进展。 使技术能够 根据这一建议，发展的能力是有选择地结合 生物活性物质到下面的化学传感器上的特定位点 芯片，在一种允许处理整个晶片的方法中 （数千个传感器）同时进行。 这将使用光反应 异双官能交联剂的方法步骤， 类似于传统的集成电路光刻，并且 仅需要对标准集成电路进行轻微修改 加工设备 用于多分析物分析的微生物传感器阵列将通过以下方式形成： 在晶片上重复固定程序，使用不同的 生物试剂，和光掩模对准，以选择不同的 固定场所。 良好控制的链接过程应该 产生具有高和可预测活性的生物分子层。 这种通用方法直接适用于检测非常宽的 通过改变固定化的生物组分来改变化合物的范围。 由此产生的生物传感器将利用自然识别， 生物试剂物种的亲和力和催化作用， 选择性化学传感器和集成电子器件。 这些 传感器将大大提高低成本直接 测量污染物、药物、激素和代谢化合物。

This project will attempt to merge microelectronics, chemical- selective membranes, and bioreagents (enzymes and antibodies) to produce integrated biosensor arrays. It will capitalize on recent advances in all three of these areas. The enabling technology to be developed under this proposal is the ability to selectively bind bioactive species to specific sites on the underlying chemical sensor chip, in a methodology which allows processing of full wafers (thousands of sensors) at once. This will be done using photoreactive heterobifunctional cross-linking reagents in process steps which are similar to conventional integrated circuit photolithography, and require only slight modifications to standard integrated-circuit processing equipment. Microbiosensor arrays for multianalyte analysis will be formed by repeating the immobilization procedure on a wafer, using different bioreagents, and a photomask aligned to select different immobilization sites. The well-controlled linking process should yield biomolecule layers which have a high and predictable activity. This general approach is directly adaptable to detecting a very wide range of compounds by varying the immobilized biological components. The resulting biosensors will utilize the natural recognition, affinity and catalysis of bioreagent species in conjunction with selective chemical transducers and integrated electronics. These sensors will significantly advance capabilities for low-cost direct measurement of pollutants, drugs, hormones and metabolic compounds.