Development of Modern Analytical Centrifugation Instruments and Methods

超速离心法可以提供有关生物大分子纯度、大小、形状和相互作用的直接、定量信息。现代大分子分析超速离心法的发展和超速离心数据分析和解释的自动化方法的发展对分子生物学的发展具有重要意义。该建议涉及的任务包括：1)完成实时重力扫描沉积速度分析的开发，包括集成基于图形的数据解释软件；2)开发分析相对于浮力质量是不均匀的分子之间的结合的方法，3)将实时干涉光学系统应用于XL-AN超速离心机。最终目标是向科学界提供能够完成水动力和热力学分析的现代、全自动化仪器和软件。

Ultracentrifugation can provide direct, quantitative information concerning the purity, size, shape and interactions of biological macromolecules. The development of a modern analytical ultracentrifuge methods for macromolecules and automated methods for the analysis and interpretation of ultracentrifugal data is important for advances in molecular biology. The tasks addressed by this proposal include: 1) completing the development of real- time, gravitational-sweep sedimentation velocity analysis, including the integration of graphics-based data interpretation software, 2) development of methods for the analysis of associations between molecules which are heterogeneous with respect to buoyant mass, and 3) adapting the real-time interference optical system to the XL-An ultracentrifuge. The ultimate goal is to provide the scientific community with a modern, fully-automated instrument and software capable of complete hydrodynamic and thermodynamic analyses.