Assembly of a Core Facility for Biological and Biochemical Research -- A Laboratory for Protein/Peptide Synthesis, Purification, and Characterization

此申请旨在寻求对 建立蛋白质/肽基本核心设施 合成、纯化和表征 格鲁吉亚理工学院化学与生物化学 技术. 该设施正在建立，以提供例行的 获得基本生物化学/生物学的主要部分 仪器仪表 私家侦探要氨基酸分析仪， 高压灭菌器，超浓缩机，高速离心机，冷 室，密度计，发酵罐，FPLC，HPLC， 蛋白质/肽测序仪，肽合成仪，和 闪烁计数器来装备这个核心设施。 用于蛋白质/肽合成、纯化 和特性将主要用于五个不同的 研究小组，他们目前可以接触到现代的 生物和生化设备要么极其有限， 或者根本不存在 这个设施将大大提高我们的能力 探讨紫外线对眼透镜的影响 蛋白质，T7功能和调节的分子基础 RNA聚合酶，突变对折叠途径的影响， 泛素，设计和合成的特异性和有效的 蛋白酶抑制剂和豌豆凝集素的晶体结构， 人血清转铁蛋白。

The purpose of this application is to solicit support for the establishment of a basic core facility for Protein/Peptide Synthesis, Purification, and Characterization in the School of Chemistry and Biochemistry at the Georgia Institute of Technology. The facility is being established to provide routine access to the major pieces of basic biochemical/biological instrumentation. The PIs are requesting an amino acid analyzer, an autoclave, an ultracentrifuge, a high speed centrifuge, a cold room, a densitometer, a fermenter, a FPLC, a HPLC, a protein/peptide sequencer, a peptide synthesizer, and a scintillation counter to equip this core facility. The core facility for protein/peptide synthesis, purification, and characterization will be used primarily by five different research groups whose present access to modern pieces of biological and biochemical equipment is either extremely limited or nonexistant. The facility will greatly enhance our capability to investigate the effects of ultraviolet radiation on eye lens proteins, the molecular basis of function and regulation of T7 RNA polymerase, the effect of mutations on the folding pathway of ubiquitin, the design and synthesis of specific and effective protease inhibitors, and the crystal structures of pea lectin and human serum transferrin.