Kinetics of Growth And Product Formation In Hairy Root Cultures

主要研究者（PI） 计划研究 生长和噻吩生产 毛状根培养 万寿菊属（万寿菊）。 噻吩是噻吩的硫衍生物， 聚乙炔，并发现主要 在这些植物的根部。 最近的研究结果表明， 可能起到保护 植物病原真菌， 昆虫和线虫。 噻吩 因此，可以提供一个 环境安全的替代品， 传统农药。 本项目的目标是 收件人： - 制定和解决 生物量数学模型 生长和噻吩 毛状根积累 万寿菊属植物的克隆 - 测定动力学参数 这些模型来自于增长数据 引发的和非引发的 万寿菊毛状根无性系 在摇瓶中培养。 - 测试各种有效性 模型比较 来自生物反应器的生长数据 生长的根，并优化 生物反应器性能， 最好的数学模型

The Principal Investigators (PIs) plan to investigate the kinetics of growth and thiophene production from fast-growing hairy root cultures of the Tagetes species (marigolds). Thiophenes are sulfur derivatives of polyacetylenes, and are found mainly in the roots of these plants. Recent findings suggest that they may play a role in protecting the plant from phytopathogenic fungi, insects, and nematodes. Thiophenes may, therefore, provide an environmentally safe alternative to conventional pesticides. The objectives of this project are to: - Formulate and solve mathematical models of biomass growth and thiophene accumulation in hairy root clones of the Tagetes species. - Determine kinetic parameters in these models from growth data of elicited and non-elicited Tagetes hairy root clones cultured in shake flasks. - Test the validity of various models by comparison with growth data from bioreactor grown roots and to optimize the bioreactor performance using the best mathematical models.