Long and Medium-Term Research: Genetic Engineering of Sterility in Plants

长期中期研究：不育基因工程 在植物中 该奖项是根据长期-中期计划 在外国卓越中心的研究，这使得 美国科学家和工程师将进行3至12次 在国外的卓越研究中心工作了几个月。 的 该计划的奖项为联合研究提供了机会， 以及使用独特或互补的设施、专业知识 国外的实验条件。 该奖项将支持为期12个月的博士后研究 饰Dr. Steven H.施特劳斯与W. J.皮科克博士和 E.S. CSIRO植物工业部的丹尼斯， 澳大利亚堪培拉，研究基因改造方法， 在植物中改造不育性， 提高农业和林业生产效率 杂交，增强营养生长， 基因工程进入自然种群。 博士 施特劳斯将重点关注生殖特异性的隔离 来自芸苔属的启动子。 合作者将使用 差减杂交技术 作为cDNA克隆分离转录物， 特别是在生殖芽发育期间。 他们将 也使用生殖特异性基因特征，在其他 物种，以方便筛选文库。 一旦他们 具有显示出足够水平的特异性的cDNA， 北方分析，它们将被用作探针来筛选 拟南芥的基因组文库，以及它们的序列 构建引物以分离核酶 （由植物工业实验室开发）， 生殖所必需的基因表达， 植物不育性 该奖项的建议提供资金，以支付国际 差旅费、家属津贴和一套行政公寓 250美元的津贴为美国家庭机构。

Long & Medium-Term Research: Genetic Engineering of Sterility in Plants This award is made under the Program for Long- & Medium-Term Research at Foreign Centers of Excellence, which enables U.S. scientists and engineers to conduct three to twelve months abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Steven H. Strauss with Drs. W.J. Peacock and E.S. Dennis of the CSIRO Division of Plant Industry, Canberra, Australia, to study methods for genetically engineering sterility in plants, which would benefit agriculture and forestry by improving efficient production of hybrids, enhancing vegetative growth, and preventing engineered genes from entering natural populations. Dr. Strauss will focus on the isolation of reproductive-specific promoters from Brassica. The collaborators will use techniques of subtractive and differential hybridization to isolate transcripts as cDNA clones that are expressed specifically during reproductive bud development. They will also use reproductive-specific genes characterized in other species to facilitate screening of libraries. Once they have cDNAs which show adequate levels of specificity in northern analyses, they will be used as probes to screen genomic libraries of Arabidopsis, and their sequences used to construct primers to enable isolation of ribozymes (developed by Plant Industry laboratories) that diminish expression of genes essential for reproduction, causing plant sterility. The award recommendation provides funds to cover international travel, dependents' allowance, and a flat administrative allowance of $250 for the U.S. home institution.