Instrumentation Development of Enhanced Capillary Zone Electrophoresis for Bioanalyses

毛细管区带电泳（CZE）可能是高效毛细管电泳（HPCE）中最简单也是最普遍使用的模式，它结合了传统电泳在分辨率上的优势和高效液相色谱（HPLC）在分析方便和速度上的优势。CZE的一个基本问题是直接控制水/毛细管界面上的zeta电位。zeta电位的定向控制对于控制毛细管内的电渗透流动和大分子在毛细管壁上的吸附是必不可少的。因此，在本研究中提出了一种仪器方法，该方法涉及使用来自毛细管外部的附加电场来控制zeta电位。这种方法的基本原理将在实验和理论上与所提出的电容器模型进行研究。在定性和定量应用中，蛋白质混合物和DNA片段的分离效率和分辨率的显著改进将得到证明和实现。

Capillary Zone Electrophoresis (CZE) is probably the simplest and perhaps the most universally useful mode of High Performance Capillary Electrophoresis (HPCE), which combines the strengths of both conventional electrophoresis in resolving power and High Performance Liquid Chromatography (HPLC) in the ease and speed of analysis. A fundamental problem in CZE is to directly control the zeta potential at the aqueous/capillary interface. Director control of the zeta potential is essential for manipulating the electroosmotic flow inside the capillary and the adsorption of macromolecules onto the capillary wall. An instrumentation method involving the use of an additional electric field from outside the capillary for controlling the zeta potential is therefore proposed in this study. The fundamentals of this approach will be studied both experimentally and theoretically with the proposed capacitor model. Significant improvements of separation efficiency and resolution of protein mixtures and DNA fragments for both qualitative and quantitative applications will then be demonstrated and achieved.