Domains of Gene Activity in Developing Embryos of Brassica napus

本研究的目的是进一步了解空间信息在高等植物胚胎发育和向萌发过渡过程中的作用。该方法将使用分子探针和原位定位技术来观察甘蓝型油菜胚胎中基因表达模式的细胞基础，并研究这些模式在发育过程中如何变化。这项研究的一个重要结果将是鉴定克隆，可以用来观察似乎涉及模式形成的过程的分子解剖学，特别是确定早期胚胎发生期间的茎尖分生组织和确定早芽发芽期间茎尖的器官身份。这些克隆还可以结合拟南芥发育突变体的遗传分析来剖析植物胚胎模式形成的机制。

The goal of the proposed research is to further our understanding of the role spatial information plays in regulating embryo development and the transition to germination in higher plants. The approach will be to use molecular probes and in situ localization techniques to look at the cellular basis for patterns of gene expression in Brassica napus embryos, and to examine how these patterns change over the course of development. An important consequence of this research will be there identification of clones that can be used to look at the molecular anatomy of the processes that appear to involve pattern formation, particularly the determination of the shoot apical meristem during early embryogenesis and the determination of organ identity at the shoot apex during precocious germination. These clones can also be used, in combination with genetic analysis of developmental mutants in Arabdopsis to dissect the mechanisms of pattern formation in plant embryos.