Regulation of Nuclear Enzymes by Phytochrome

这个项目的目的是了解更多关于结构， 豌豆核中酶的功能和调节， 受到光敏色素的刺激 光敏色素能刺激 纯化的豌豆核中的核苷三磷酸酶（NTH），和 钙离子是这种反应所必需的。 一种钙调素- 依赖性的47 kD NTR已从豌豆核中分离出来， 具有暗示它是核NTT的特性 这是由光敏色素调节的。 这个NTT似乎是 与哺乳动物核纤层蛋白衍生的46 kD NTR密切相关 细胞核，其活性被认为对RNA输出很重要 从原子核。 关于植物NTR的其他序列信息 来证实或反驳这种关系。 那里 有证据表明，动物核纤层蛋白的磷酸化 原子核在其受控降解中起着关键作用， 蛋白水解 豌豆NTR的磷酸化将是 特点，其作用都在调节NTT 活性和从层粘连蛋白样前体生产NTR 将被审查。 这些结果应有助于改善 了解光如何影响核代谢， 也可能对光如何调节植物的模型产生影响 基因表达。//

The objective of this project is to learn more about the structure, function and regulation of enzymes in pea nuclei that are stimulated by phytochrome. Phytochrome stimulates the activity of nucleoside triphosphatase (NTPase) in purified pea nuclei, and calcium ions are necessary for this response. A calmodulin- dependent 47 kD NTPase has been isolated from pea nuclei and shown to have properties that implicate it as being the nuclear NTPase that is regulated by phytochrome. This NTPase appears to be closely related to a lamin-derived 46 kD NTPase in mammalian nuclei, whose activity is thought to be important for RNA export from nuclei. Additional sequence information on the plant NTPase will be obtained to confirm or disprove this relationship. There is some evidence that the phosphorylation of lamins in animal nuclei plays a critical role in its controlled degradation by proteolysis. The phosphorylation of the pea NTPase will be characterized, and its role both in the regulation of NTPase activity and in the production of NTPase from lamin-like precursors will be examined. The results should contribute to an improved understanding of how light can influence nuclear metabolism, with possible implications also for models of how light regulates plant gene expression.//