Development of an Efficient Transformation-Regeneration System for Soybean (Glycine max)

在过去的十年中，选定的植物物种的转化再生已经成为常规，并有助于通过基因工程生产改良的植物产品。然而，尽管做出了巨大的努力，但还没有为谷物和豆类这两种经济上最重要的植物类群开发出有效的转化-再生系统。本项目以大豆为示范试验系统，为开发这样一个豆类系统进行了全面和系统的努力。研究小组是大豆组织培养和遗传学专家，也是细胞生物学专家，他们将通过鉴定参与再生的细胞，开发转化技术。如果成功，这种技术将直接有助于豆科植物的基因工程，并间接有助于我们对植物转化基本机制的理解。

During the past decade, the transformation-regeneration of selected plant species has become routine and contributed to the production of improved plant products through genetic engineering. However, despite a large effort, an efficient system for transformation- regeneration has not been developed for the two most economically important groups of plants, cereals and legumes. This project represents a comprehensive and systematic effort to develop such a system for legumes, using soybean as a model experimental system. The investigators, an expert on soybean tissue culture and genetics and an expert in cell biology, will develop a transformation technology by identifying the cells participating in regeneration. If successful, such a technology will contribute directly to the genetic engineering of legumes, and indirectly to our understanding of the basic mechanisms of plant transformation.