Molecular Genetic Techniques of Undergraduates

9451047摩尔本提案的目的是为最先进的分子遗传学技术课程配备一个教学实验室。 分子遗传学在生物学研究的大多数领域正成为越来越重要的工具。学生获得分子遗传学知识和分子遗传技术的实践经验是至关重要的。本课程旨在向学生介绍分子遗传技术的理论和基本生物学原理。 学生还将接触到利用分子遗传技术领域的各种研究项目和就业机会。此外，学生将获得各种最新分子遗传技术的实践经验。本课程的实验室部分将集中在一个单一的有机体，盘基网柄藻，以使学生有机会获得一些深入的了解生物系统上的实验正在进行，并分析他们收集的数据在一个有意义的方式。除了实验室实验，学生将学习如何访问和使用计算机分子遗传学数据库。 在实验室接触计算机是必不可少的，因为计算机成为生物研究的一个组成部分。除了在课程中学到的材料和技术，增加接触分子遗传学和其他实验室技术，沿着现代实验室设备的可用性，将鼓励学生参与独立研究，无论是与教师在跨西尔瓦尼亚大学或其他研究机构。所需的主要仪器包括海底琼脂糖凝胶电泳、聚丙烯酰亚胺凝胶电泳、2套DNA测序凝胶电泳系统、1台热循环仪和4台配有适当软件和附件的微型计算机所需的设备。

9451047 Moore The objective of this proposal is to equip a teaching laboratory for a state-of-the-art molecular genetics techniques course. Molecular genetics is becoming an increasingly important tool in most areas of biological research. It is critical that students have access to knowledge of molecular genetics and practical experience in molecular genetic techniques. This course is designed to introduce the students to the theory and basic biological principles underlying molecular genetic techniques. Students will also be exposed to a variety of research programs and job opportunities in areas utilizing molecular genetic techniques. In addition, students will gain practical experience in a variety of up-to-date molecular genetic techniques. The laboratory component of the course will focus on a single organism, Dictyostelium discoideum, in order to give the students the opportunity to gain some in-depth knowledge about the biological system on which the experiments are being carried out and to analyze the data they collect in a meaningful way. In addition to the laboratory experiments, the students will learn how to access and use computer molecular genetics data bases. Exposure to computers in the laboratory is essential as computers become an integral part of biological research. In addition to the material and techniques learned during the course, increased exposure to molecular genetics and other laboratory techniques, along with the availability of modern laboratory equipment, will encourage students to participate in independent research either with the faculty at Transylvania University or at other research institutions. Major instruments requested include equipment necessary for submarine agarose gel electrophoresis, polyacrylimide gel electrophoresis, 2 DNA sequencing gel electrophoresis systems, a thermocycler, and 4 microcomputers with appropriate software and accessories.