Molecular Genetic Techniques of Undergraduates

9451047摩尔这项建议的目的是为一门最先进的分子遗传学技术课程配备一个教学实验室。在生物学研究的大多数领域，分子遗传学正成为一个日益重要的工具。让学生掌握分子遗传学知识和分子遗传学技术的实践经验是至关重要的。本课程旨在向学生介绍分子基因技术背后的理论和基本生物学原理。学生还将接触到利用分子基因技术领域的各种研究项目和工作机会。此外，学生将在各种最新的分子遗传学技术方面获得实践经验。本课程的实验部分将侧重于一种单一的生物体-盘基网柄菌，以使学生有机会对正在进行实验的生物系统获得一些深入的知识，并以有意义的方式分析他们收集的数据。除了实验室实验，学生还将学习如何访问和使用计算机分子遗传学数据库。随着计算机成为生物学研究不可或缺的一部分，接触实验室中的计算机是必不可少的。除了在课程中学到的材料和技术外，增加对分子遗传学和其他实验室技术的接触，以及现代实验室设备的可获得性，将鼓励学生与特兰西瓦尼亚大学的教员或其他研究机构一起参与独立研究。所需的主要仪器包括海底琼脂糖凝胶电泳仪、聚丙烯酰亚胺凝胶电泳仪、2套DNA测序凝胶电泳仪、1台热循环仪和4台装有适当软件和附件的微型计算机。

9451047 Moore The objective of this proposal is to equip a teaching laboratory for a state-of-the-art molecular genetics techniques course. Molecular genetics is becoming an increasingly important tool in most areas of biological research. It is critical that students have access to knowledge of molecular genetics and practical experience in molecular genetic techniques. This course is designed to introduce the students to the theory and basic biological principles underlying molecular genetic techniques. Students will also be exposed to a variety of research programs and job opportunities in areas utilizing molecular genetic techniques. In addition, students will gain practical experience in a variety of up-to-date molecular genetic techniques. The laboratory component of the course will focus on a single organism, Dictyostelium discoideum, in order to give the students the opportunity to gain some in-depth knowledge about the biological system on which the experiments are being carried out and to analyze the data they collect in a meaningful way. In addition to the laboratory experiments, the students will learn how to access and use computer molecular genetics data bases. Exposure to computers in the laboratory is essential as computers become an integral part of biological research. In addition to the material and techniques learned during the course, increased exposure to molecular genetics and other laboratory techniques, along with the availability of modern laboratory equipment, will encourage students to participate in independent research either with the faculty at Transylvania University or at other research institutions. Major instruments requested include equipment necessary for submarine agarose gel electrophoresis, polyacrylimide gel electrophoresis, 2 DNA sequencing gel electrophoresis systems, a thermocycler, and 4 microcomputers with appropriate software and accessories.