E. coli Colony Development

表面生长是自然界中细菌作用的一个关键特征。 的 E.大肠杆菌菌落是一个很好的细菌表面模型系统 人口。 这是一个高度组织化的结构，其特点是 差异基因表达 细胞间相互作用 微米的距离调节单个细胞的生长， 基因表达模式可以在种群之间协调 以毫米的距离分开。 扩散信号影响 殖民地发展的几个方面。遗传位点学科 在菌落发育过程中的差异控制已被确定 通过自发和转座子插入突变以及使用 lacZ融合。 此外，cAMP系统还与 激活Mudiac元件的空间定位复制。 实验计划将通过以下方式扩展这些初步观察结果： 详细研究了三个系统：（1）发育调节 dev1891：：lacZ fusion。 (2)DNA聚合酶1的作用， 系统和Mudiac控制中的扩散物质 复制，和（3）dev2137和dev2137突变位于 染色体的oriC区域。 结果将指定基因 涉及菌落形态发生的产品，提供了更详细的 在琼脂上生长期间差异基因表达的图片 表面，并识别影响菌落的可扩散信号 发展理念

Surface growth is a key feature of bacterial action in nature. The E. coli colony is an excellent model system of bacterial surface populations. It is a highly organized structure characterized by differential gene expression. Cell-cell interactions over distances of microns regulate the growth of individuals cells, and gene expression patterns can be coordinated between populations separated by distances of millimeters. Diffusible signals affect several aspects of colony development. Genetic loci subject differential control during colony development have been identified by spontaneous and transposon insertion mutations and the use of lacZ fusions. In addition, the cAMP system has been implicated in activating the spatially localized replication of Mudiac elements. The experimental program will extend these initial observations by detailed study of three systems:(1) developmental regulation of the dev1891::lacZ fusion. (2) the role of DNA polymerase 1, the cAMP system, and diffusible substances in the control of Mudiac replication, and (3) the dev2137 and dev2137 mutations located in the oriC region of the chromosome. The results will specify gene products involved in colony morphogenesis, provide a more detailed picture of differential gene expression during growth on an agar surface, and identify diffusible signals that affect colony developments.