Acquisition of a Centrifuge, HPLC System and UV/Visible Spectrophotometer

在过去的几年里，迈阿密生化分部的研究活动水平大幅提高。该部门增加了一名新教员(一个新职位)，并获得了97.5万美元的研究资金，其中包括来自国家科学基金会的四项拨款。研究活动的增加查明了各司在工具方面的若干不足之处。几个受资助的研究项目需要该司目前没有的能力。所需的仪器包括：一个超高速离心机和转子，一个具有微孔能力的梯度高效液相色谱系统，用于分离ug数量的蛋白质，以及一个高灵敏度的扫描紫外/可见光分光光度计。这三件设备通常在生化实验室中找到，对至少两个私人投资机构来说是必不可少的，该部门的其他三个小组偶尔也会使用这些设备。所要求的仪器将用于支持和加强以下研究项目：1)植物线粒体生物发生和花粉发育的发育调控，2)植物脂肪酸的代谢调控和生物合成，以及3)单宁在植物-食草动物相互作用中的作用。

In the last several years the level of research activityof the Miami Biochemistry division has increased dramatically. The division has added a new faculty member (a new position) and obtained $975,000 in research funding including four grants from NSF. This increase in research activity has identified several inadequacies in the divisions' instrumentation. Several o fthe funded research projects require capabilities not currently available in the division. The required instrumentation are: a supraspeed centrifuge and rotors, a gradient HPLC system with microbore capabilities to isolate ug quantitites of protein, and a scanning UV/visibile spectrophotometer with high sensitivity. All three pieces of equipment, commonly found in biochemistry laboratories, are essential to at least two of the PIs and will be used occasionally by the other three groups in the division. The requested instruments will be used to support and enhance the following research projects: 1) development regulation of plant mitochondrial biogenesis and pollen development, 2) metabolic regulation and biosynthesis of fatty acids in plant, and 3) the role of tannins in plant-herbivore interactions.