Manipulation of Cell Behavior in Three-Dimensional Culture

该项目的目标是开发方法， 操纵三维凝胶中的细胞行为。 要做到这一点，就必须系统地研究 人白细胞和PC12细胞的细胞行为 分散在胶原凝胶中。 具体来说，Cell 胶原蛋白的行为（即运动性和神经突生长） 具有不同水合作用的凝胶，胶原蛋白中的细胞行为 具有添加的细胞外基质（ECM）组分的凝胶，和 具有附加生物反应的凝胶中的细胞行为 修改器将被检查。 通过控制释放 聚合物作为生物活性剂（抗体， 生长因子、趋化因子和ECM分子）， 在充分表征的浓度下的细胞行为 梯度也要量化。

The goal of this project is to develop methods for manipulating cell behavior in three-dimensional gels. This is to be achieved by a systematic study of changes in cell behavior for human leukocytes and PC12 cells dispersed within collagen gels. Specifically, cell behavior (i.e. motility and neurite outgrowth) in collagen gels with different hydration, cell behavior in collagen gels with added extracellular matrix (ECM) components, and cell behavior in gels with added biological response modifiers will be examined. By using controlled release polymers as sources of bioactive agents (antibodies, growth factors, chemotactic factors,and ECM molecules), cell behavior in well characterized concentration gradients is also to be quantified.