Introduction of Ultracentrifugation into the Cell Biology Curriculum

格林内尔学院的生物系正在 修订其细胞生物学的副教授级核心课程。在 过去，实验室由一系列独立的 在练习中学习了重要的技术， 经验并没有以任何方式统一。我们建议发展 一系列的连续实验， 细胞的结构和功能， 细胞器的分离。实验室计划 从植物和动物组织中制备用于研究的细胞器 某些细胞器的活动，如氧气 消耗的孤立线粒体，电子流在孤立的 叶绿体，不同标记酶的动力学研究 细胞组分、组织和蛋白质多样性， 分化组织 所有必要的设备 实验室是可用的，除了一个 超级电容器和转子。我们还提出了更新的实验 在四个高级课程中， 制备血浆研究组分所必需的 膜功能，质粒分离，病毒纯化 颗粒和通过电子显微镜检查级分。 加入一个超级电容器， 扩大学生的第一手经验 用现代生物仪器。

The Department of Biology at Grinnell College is in the process of revising its sophomore-level core course in Cell Biology. In the past the laboratory consisted of a series of independent exercises where important techniques were learned but the experience was not unified in any way. We propose development of a series of sequential experiments unified around the study of cellular structure and function, made possible by fractionation of organelles. Laboratories are planned to prepare organelles from plant and animal tissues for the study of the activities of selected organelles such as oxygen consumption by isolated mitochondria, electron flow in isolated chloroplasts, kinetic studies of marker enzymes for different cellular fractions, organization, and protein diversity in differentiated tissues. All equipment necessary for these laboratories is available with the exception of an ultracentrifuge and rotors. We also propose updated experiments in four upper-level courses where ultracentrifugation will be necessary for preparation of fractions for the study of plasma membrane function, plasmid isolation, purification of virus particles and examination of fractions by electron microscopy. The addition of an ultracentrifuge will make it possible for the department to broaden the firsthand experience of students with modern biological instrumentation.