Measuring Metabolic Fluxes: Applications for Elucidating Energetics and Regulation

该项目计划绘制 通量，酶活性，和 关键反应的代谢水平 在糖酵解途径中， 三羧酸（TCA）循环 两种常见细菌E. coli和B. 枯草杆菌。 野生型和 将检测代谢突变体 为了获得监管 洞察力和替代产量 场景 预计将有四个领域 受到影响。 第一，改进的 了解三氯乙酸循环 和糖酵解是协调的 将获得。 二是 能量溢出的大小 反应将被确定。 第三，结果可以证明 有利于发展种植业 促进最大化的条件 将碳转化为细胞质量。 最后，代谢工程 可以确定战略， 可以让人改变 调节细胞在一个 最佳方式。

This project plans to map the fluxes, enzyme activities, and metabolic levels for key reactions in the glycolytic pathway and the tricarboxylic acid (TCA) cycle for two common bacteria, E. coli and B. subtilis. Both wild-type and metabolic mutants will be examined in order to obtain regulatory insights and alternative yield scenarios. Four areas are expected to be impacted. First, an improved understanding of how the TCA cycle and glycolysis are coordinated would be obtained. Secondly, the magnitude of the energy spilling reactions would be determined. Thirdly, the results could prove useful to developing cultivation conditions that promote the maximal conversion of carbon to cell mass. Finally, metabolic engineering strategies could be identified that would allow one to alter the regulation of the cell in an optimal manner.