Development of 750 MHz Spectroscopy at the National Magnetic Resonance Facility at Madison.

申请的资金为布鲁克AMX-750的50%资金 （17.63特斯拉，750兆赫）核磁共振光谱仪，这将成为 计划投资3000万美元扩建生物化学 美国威斯康星大学麦迪逊分校 的一翼 计划中的建筑扩建旨在满足当前 以及国家磁共振设备的未来需求 在麦迪逊（NMRFAM）。 这一翼将与 使用的当前共享仪表设施 物理和生物科学的研究人员， 威斯康星大学麦迪逊分校和游客从 美国的其他研究机构和一些 国外 750 MHz NMR光谱仪代表 新兴技术。 它将包含一个持久的 17.63特斯拉超导螺线管与5厘米室- 1991年由布鲁克公司开发的温度孔。 与该系统相关的控制台和探头将 最先进的代表。新的 容纳该仪器的结构将被设计为 处理本系统的特殊选址要求。 750 MHz NMR光谱仪的研究时间将在 根据科学价值分配给竞争对手， 项目 地方和国家咨询委员会， 全国小额信贷和家庭管理研究所将监督其使用情况。 国家医疗和康复管理委员会有全职支持 工作人员（包括四名具有博士学位的设施工作人员） 将提供：用户培训; 光谱数据采集、处理和分析; 日常维护和维修。 NMRFAM也有一个证明 向其提供新技术的跟踪记录 用户，用于保持仪器处于最高运行状态 条件，用于修改或构造特殊电路 和探针，并为NMR数据创建软件工具 分析. 750 MHz NMR光谱仪的用户群 包括研究生，博士后研究， 助理、科学家、主要研究者表格 40个实验室在15个部门对大学 威斯康星-麦迪逊校区。 此外，该设施还用于 大约有35个组织在校外。 新大楼 此外，还将为来访的科学家提供空间。 的750 MHz频谱仪将用于需要 其极高的磁场强度： 大生物分子中的Overhauser效应， 化学交换和关系的场依赖性 现象、蛋白质的功能研究和其他 需要特殊分辨率的生物分子和/或 灵敏度 加上这个光谱仪， NMRFAM将处于独特的地位，为用户提供 磁场强度在9.4和17.63特斯拉之间， 场相关现象的研究。 全国小额贷款和家庭援助机构的工作人员 将开发核磁共振实验方案，以处理 问题和机会，这一新的光谱仪将 提供结构和机械方面的研究和培训 生物学

Funds are requested for 50% funding of a Bruker AMX-750 (17.63 tesla, 750 MHz) NMR spectrometer which will become part of a $30 million planned expansion of the Biochemistry Department, University of Wisconsin-Madison. One wing of the planned building expansion is designed to meet current and future needs of the National Magnetic Resonance Facility at Madison (NMRFAM). This wing will be contiguous with the current shared instrumentation facility which is used by researchers in the physical and biological sciences at the University of Wisconsin-Madison campus and visitors from other research institutions in the United States and several foreign countries. The 750 MHz NMR spectrometer represents newly emerging technology. It will incorporate a persistent 17.63 Tesla superconducting solenoid with 5-cm room- temperature bore of the type developed by Bruker in 1991. The console and probes associated with this system will be representative of the state-of-the-art. The new construction to house this instrument will be designed to handle the special siting requirements of this system. Research time on the 750 MHz NMR spectrometer will be allocated on the basis of scientific merit to competing projects. The local and national advisory committees to NMRFAM will oversee its use. NMRFAM has full-time support staff (including four facility staff members with Ph.D. degrees) who will provide: user training; assistance with spectral data acquisition, processing, and analysis; routine maintenance; and repairs. NMRFAM also has a proven track record for making new technology available to its users, for keeping instrumentation in top operating condition, for modifying or constructing special circuits and probes, and for creating software tools for NMR data analysis. The user base for the 750 MHz NMR spectrometer includes graduate students, postdoctoral research associates, staff scientists, principal investigators form 40 laboratories in 15 departments on the University of Wisconsin-Madison Campus. In addition, the facility is used by about 35 groups located off campus. The new building addition will have space for visiting scientists. The 750 MHz spectrometer will be devoted to experiments that require its uniquely-high field strength: studies of the nuclear Overhauser effect in larger biomolecules, studies of the field dependence of chemical exchange and relation phenomena, functional studies of proteins and other biomolecules that require exceptional resolution and/or sensitivity. With the addition of this spectrometer, NMRFAM will be in the unique position of offering its user magnetic field strengths between 9.4 and 17.63 tesla for studies of field -dependent phenomena. The staff of NMRFAM will develop NMR experimental protocols to deal with the problems and opportunities that this new spectrometer will offer to research and training in structural and mechanistic biology.