Acquisition of a Mass Spectrometer

9217434 Matsuaira A质谱仪将主要由参与蛋白质测序和多肽合成的团体使用，这些团体目前使用外部设施进行质量分析，并被小鼠基因组项目用于开发绘制dna片段图谱的方法。激光解吸是检测、分辨和测定分子质量的一种非常优越的技术。激光解吸电离样品比快原子轰击电离、等离子体解吸、电喷雾或热喷雾电离有许多优点。Lasermat通常提供1000-100,000范围内的质量，从1 pmoL的蛋白质和多肽。质量观测质量的准确度依赖于样本，但在质量为30,000的情况下通常在0.1%以内。该仪器通过个人计算机上易于使用的软件进行操作，该软件控制数据收集和分析。该软件简化了非专业研究人员对仪器操作的培训，并消除了对质谱学专家操作仪器和分析光谱的需要。

9217434 Matsudaira A mass spectrometer will be used primarily by groups involved in protein sequencing and peptide synthesis that currently use outside facilities for mass analysis and by the mouse genome project for developing methods for mapping DNA fragments. Laser desorption is a far superior technique for detecting, resolving, and determining the mass of a molecule. Ionization of the sample by laser desorption has many advantages over ionization by fast atom bombardment, plasma desorption, electrospray, or thermospray methods. The Lasermat routinely provides masses in the range 1,000 - 100,000 from 1 pmol of proteins and peptides. The accuracy of the mass observed mass is sample dependent but is generally within 0.1% at a mass of 30,000. The instrument is operated through easy-to-use software on a personal computer which controls data collection and analysis. The software simplifies the training of unspecialized researchers in the operation of the instrument and eliminates the need for a specialist in mass spectrometry to operate the instrument and analyze the spectra.