Molecular Genetics of Disease Resistance in Tomato

对丁香假单胞菌的耐药性。番茄（Pst）是由一个显性位点Pto赋予的。克隆基因座所需的工具是在植物博士后奖学金的任期内开发的。利用番茄近等基因系鉴定与该基因紧密相连的RFLP和RAPD标记，并利用大量F2群体建立高分辨率的连锁图谱，从而实现Pto分子标记的精确定位。构建番茄YAC文库，筛选Pto位点候选基因。该项目将继续进行，并对Pto基因进行分离和鉴定。这项工作将大大提高我们对植物病害控制的认识和能力。

Resistance to Pseudomonas syringae pv.tomato(Pst) in tomato is conferred by a single dominant locus, Pto. Tools necessary for cloning the locus were developed during the tenure of the Plant Postdoctoral Fellowship Award. Near Isogenic lines of tomato were used to identify RFLP and RAPD markers tightly linked to the gene and a large F2 population was used to create a high resolution linkage map that allowed precise placement of Pto in relation to the molecular markers. A tomato YAC library was constructed and a screen identified a candidate for the Pto locus. The project will be continued and the putative Pto gene isolated and characterized. %%% This work should significantly increase our understanding and ability to control disease in plants.